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A Kinesin-14 Motor Activates Neocentromeres to Promote Meiotic Drive in Maize.
Dawe, R Kelly; Lowry, Elizabeth G; Gent, Jonathan I; Stitzer, Michelle C; Swentowsky, Kyle W; Higgins, David M; Ross-Ibarra, Jeffrey; Wallace, Jason G; Kanizay, Lisa B; Alabady, Magdy; Qiu, Weihong; Tseng, Kuo-Fu; Wang, Na; Gao, Zhi; Birchler, James A; Harkess, Alex E; Hodges, Amy L; Hiatt, Evelyn N.
Affiliation
  • Dawe RK; Department of Plant Biology, University of Georgia, Athens, GA 30602, USA; Department of Genetics, University of Georgia, Athens, GA 30602, USA. Electronic address: kdawe@uga.edu.
  • Lowry EG; Department of Genetics, University of Georgia, Athens, GA 30602, USA.
  • Gent JI; Department of Plant Biology, University of Georgia, Athens, GA 30602, USA.
  • Stitzer MC; Department of Plant Sciences and Center for Population Biology, University of California, Davis, CA 95616, USA.
  • Swentowsky KW; Department of Plant Biology, University of Georgia, Athens, GA 30602, USA.
  • Higgins DM; Department of Plant Biology, University of Georgia, Athens, GA 30602, USA.
  • Ross-Ibarra J; Department of Plant Sciences and Center for Population Biology, University of California, Davis, CA 95616, USA; Genome Center, University of California, Davis, CA 95616, USA.
  • Wallace JG; Department of Crop and Soil Sciences, University of Georgia, Athens, GA 30602, USA.
  • Kanizay LB; Department of Plant Biology, University of Georgia, Athens, GA 30602, USA.
  • Alabady M; Department of Plant Biology, University of Georgia, Athens, GA 30602, USA.
  • Qiu W; Department of Physics, Oregon State University, Corvallis, OR 97331, USA.
  • Tseng KF; Department of Physics, Oregon State University, Corvallis, OR 97331, USA.
  • Wang N; Department of Plant Biology, University of Georgia, Athens, GA 30602, USA.
  • Gao Z; Division of Biological Sciences, University of Missouri, Columbia, MO 65211, USA.
  • Birchler JA; Division of Biological Sciences, University of Missouri, Columbia, MO 65211, USA.
  • Harkess AE; Department of Plant Biology, University of Georgia, Athens, GA 30602, USA.
  • Hodges AL; Department of Plant Biology, University of Georgia, Athens, GA 30602, USA.
  • Hiatt EN; Kentucky Wesleyan College, Owensboro, KY 42301, USA.
Cell ; 173(4): 839-850.e18, 2018 05 03.
Article in En | MEDLINE | ID: mdl-29628142
ABSTRACT
Maize abnormal chromosome 10 (Ab10) encodes a classic example of true meiotic drive that converts heterochromatic regions called knobs into motile neocentromeres that are preferentially transmitted to egg cells. Here, we identify a cluster of eight genes on Ab10, called the Kinesin driver (Kindr) complex, that are required for both neocentromere motility and preferential transmission. Two meiotic drive mutants that lack neocentromere activity proved to be kindr epimutants with increased DNA methylation across the entire gene cluster. RNAi of Kindr induced a third epimutant and corresponding loss of meiotic drive. Kinesin gliding assays and immunolocalization revealed that KINDR is a functional minus-end-directed kinesin that localizes specifically to knobs containing 180 bp repeats. Sequence comparisons suggest that Kindr diverged from a Kinesin-14A ancestor ∼12 mya and has driven the accumulation of > 500 Mb of knob repeats and affected the segregation of thousands of genes linked to knobs on all 10 chromosomes.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Plant Proteins / Centromere / Kinesins / Zea mays / Meiosis Language: En Journal: Cell Year: 2018 Type: Article
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Plant Proteins / Centromere / Kinesins / Zea mays / Meiosis Language: En Journal: Cell Year: 2018 Type: Article