NIR-emitting benzothiazolium cyanines with an enhanced stokes shift for mitochondria imaging in live cells.
Org Biomol Chem
; 16(18): 3382-3388, 2018 05 09.
Article
in En
| MEDLINE
| ID: mdl-29670968
ABSTRACT
A series of benzothiazolium-based hemicyanines (3a-3f) have been synthesized. Evaluation of their photophysical properties shows that they exhibit improved photophysical characteristics. In comparison with the available commercial MitoTrackers, the new probes revealed an enhanced Stokes shift (Δλ â¼ 80 nm) and minimized aggregation for increased sensitivity. The synthesized probes are found to exhibit excellent selectivity for mitochondrial staining in an oligodendrocyte cell line. Probes show almost no fluorescence in aqueous environments, while the fluorescence is increased by â¼10-fold in organic solvents, making it possible for mitochondrial imaging without the need for post-staining washing. Since the absorption peaks of probes are close to the laser wavelengths of 561 and 640 nm on a commercial confocal microscope, e.g.3a exhibits λabs â¼ 620 nm and λem â¼ 702 nm, they could be useful probes for mitochondrial tracking in live cells.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Carbocyanines
/
Oligodendroglia
/
Benzothiazoles
/
Optical Imaging
/
Fluorescent Dyes
/
Mitochondria
Limits:
Humans
Language:
En
Journal:
Org Biomol Chem
Journal subject:
BIOQUIMICA
/
QUIMICA
Year:
2018
Type:
Article
Affiliation country:
United States