Genetic and substrate-level modulation of Bacillus subtilis physiology for enhanced extracellular human interferon gamma production.

Kumar, Nitin; Pandey, Rajat; Prabhu, Ashish Anand; Venkata Dasu, Veeranki

Kumar, Nitin; Pandey, Rajat; Prabhu, Ashish Anand; Venkata Dasu, Veeranki.

Affiliation

Kumar N; a Biochemical Engineering Laboratory, Department of Biosciences and Bioengineering , Indian Institute of Technology Guwahati , Guwahati , Assam , India.
Pandey R; a Biochemical Engineering Laboratory, Department of Biosciences and Bioengineering , Indian Institute of Technology Guwahati , Guwahati , Assam , India.
Prabhu AA; a Biochemical Engineering Laboratory, Department of Biosciences and Bioengineering , Indian Institute of Technology Guwahati , Guwahati , Assam , India.
Venkata Dasu V; a Biochemical Engineering Laboratory, Department of Biosciences and Bioengineering , Indian Institute of Technology Guwahati , Guwahati , Assam , India.

Prep Biochem Biotechnol ; 48(5): 391-401, 2018 May 28.

Article in En | MEDLINE | ID: mdl-29688129

Subject(s)

Bacillus subtilis/genetics; Cloning, Molecular/methods; Interferon-gamma/genetics; Bacillus subtilis/growth & development; Bacillus subtilis/metabolism; Codon; Genetic Engineering/methods; Humans; Industrial Microbiology/methods; Interferon-gamma/metabolism; Plasmids/genetics; Plasmids/metabolism; Recombinant Proteins/genetics; Recombinant Proteins/metabolism; Transformation, Bacterial

Key words

Acetate; PTS/non-PTS carbon sources; acetoin; codon optimization; overflow metabolism; physiological performance; soluble human interferon gamma

Fulltext

XML

PubMed Links

Search on Google

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Bacillus subtilis / Interferon-gamma / Cloning, Molecular Limits: Humans Language: En Journal: Prep Biochem Biotechnol Journal subject: BIOQUIMICA / BIOTECNOLOGIA Year: 2018 Type: Article Affiliation country: India

Fulltext

XML

PubMed Links

Search on Google