Lethal (2) giant larvae regulates pleural mesothelial cell polarity in pleural fibrosis.
Biochim Biophys Acta Mol Cell Res
; 1865(9): 1201-1210, 2018 09.
Article
in En
| MEDLINE
| ID: mdl-29842893
ABSTRACT
Pleural fibrosis is barely reversible and the underlying mechanisms are poorly understood. Pleural mesothelial cells (PMCs) which have apical-basal polarity play a key role in pleural fibrosis. Loss of cell polarity is involved in the development of fibrotic diseases. Partition defective protein (PAR) complex is a key regulator of cell polarity. However, changes of PMC polarity and PAR complex in pleural fibrosis are still unknown. In this study, we observed that PMC polarity was lost in fibrotic pleura. Next we found increased Lethal (2) giant larvae (Lgl) bound with aPKC and PAR-6B competing against PAR-3A in PAR complex, which led to cell polarity loss. Then we demonstrated that Lgl1 siRNA prevented cell polarity loss in PMCs, and Lgl1 conditional knockout (ER-Cre+/-Lgl1flox/flox) attenuated pleural fibrosis in a mouse model. Our data indicated that Lgl1 regulates cell polarity of PMCs, inhibition of Lgl1 and maintenance of cell polarity in PMCs could be a potential therapeutic treatment approach for pleural fibrosis.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Pleura
/
Glycoproteins
/
Epithelial Cells
Limits:
Animals
/
Female
/
Humans
/
Male
Language:
En
Journal:
Biochim Biophys Acta Mol Cell Res
Year:
2018
Type:
Article
Affiliation country:
China