Well-designed dopamine-imprinted polymer interface for selective and quantitative dopamine detection among catecholamines using a potentiometric biosensor.
Biosens Bioelectron
; 117: 810-817, 2018 Oct 15.
Article
in En
| MEDLINE
| ID: mdl-30096735
ABSTRACT
We report a well-designed biointerface enabling the selective and quantitative detection of dopamine (DA) using a potentiometric biosensor. To enhance the detection selectivity of DA, a DA-templated molecularly imprinted polymer (DA-MIP) was synthesized on the extended Au gate electrode of a field-effect transistor (FET) biosensor. For a quantitative DA analysis, the thickness of the DA-MIP was controlled to ca. 60â¯nm by surface-initiated atom transfer radical polymerization (SI-ATRP). In this process, the DA-MIP was copolymerized with vinylphenylboronic acid (vinyl-PBA), inducing molecular charges at the biointerface of the FET gate electrode. These charges were generated by the diol-binding between PBA and dopamine (a catecholamine), and were directly detected as a change in surface potential. In fact, the surface potential at the gate of the DA-MIP-coated FET responded significantly to DA added at concentrations ranging from 40â¯nM to 20⯵M, whereas that of a non-imprinted polymer (NIP)-coated FET hardly changed over this range. Moreover, by measuring the kinetic parameters and electrochemical properties of well-designed devices with various added catecholamines, we confirmed that the DA-MIP-coated FET biosensor selectively and quantitatively detects DA.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Polymers
/
Biosensing Techniques
/
Dopamine
Type of study:
Diagnostic_studies
Language:
En
Journal:
Biosens Bioelectron
Journal subject:
BIOTECNOLOGIA
Year:
2018
Type:
Article