Label-free real-time detection of biotinylated bovine serum albumin using a low-cost optical cavity-based biosensor.
Opt Express
; 26(15): 18982-18989, 2018 Jul 23.
Article
in En
| MEDLINE
| ID: mdl-30114158
ABSTRACT
We have developed a low-cost optical cavity-based biosensor with a differential detection method for point-of-care medical diagnostics. To experimentally demonstrate its label-free real-time biosensing capability, we performed the detection of biotinylated bovine serum albumin (BSA). Streptavidin is introduced into the optical cavity structure and immobilized on 3-aminopropyltriethoxysilane (APTES) coated surface. After rinsing out unbound streptavidin with DI water, biotinylated BSA without any labeling is introduced. A CMOS camera captures the transmitted light of two different wavelengths passing through the optical cavity sensing area in real-time. Then, the differential values are calculated to enhance the responsivity. We successfully demonstrated the label-free real-time detection of biotinylated BSA, and the measurement results matched well with the simulation results. The limit of detection of the optical cavity-based biosensor for the biotinylated BSA detection with the sensing area of 180 µm × 180 µm is estimated to be 2.82 pM, which could be reduced further for a smaller sensing area with the tradeoff of a longer sensing time.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Immunoglobulin G
/
Serum Albumin, Bovine
/
Biosensing Techniques
Type of study:
Diagnostic_studies
/
Health_economic_evaluation
Limits:
Animals
Language:
En
Journal:
Opt Express
Journal subject:
OFTALMOLOGIA
Year:
2018
Type:
Article