Reconstitution of a 26-Subunit Human Kinetochore Reveals Cooperative Microtubule Binding by CENP-OPQUR and NDC80.

Pesenti, Marion E; Prumbaum, Daniel; Auckland, Philip; Smith, Charlotte M; Faesen, Alex C; Petrovic, Arsen; Erent, Muriel; Maffini, Stefano; Pentakota, Satyakrishna; Weir, John R; Lin, Yu-Chih; Raunser, Stefan; McAinsh, Andrew D; Musacchio, Andrea

Pesenti, Marion E; Prumbaum, Daniel; Auckland, Philip; Smith, Charlotte M; Faesen, Alex C; Petrovic, Arsen; Erent, Muriel; Maffini, Stefano; Pentakota, Satyakrishna; Weir, John R; Lin, Yu-Chih; Raunser, Stefan; McAinsh, Andrew D; Musacchio, Andrea.

Affiliation

Pesenti ME; Department of Mechanistic Cell Biology, Max Planck Institute of Molecular Physiology, Otto-Hahn-Straße 11, 44227 Dortmund, Germany.
Prumbaum D; Department of Structural Biochemistry, Max Planck Institute of Molecular Physiology, Otto-Hahn-Straße 11, 44227 Dortmund, Germany.
Auckland P; Centre for Mechanochemical Cell Biology, Warwick Medical School, The University of Warwick, Coventry, CV4 7AL, UK.
Smith CM; Department of Mechanistic Cell Biology, Max Planck Institute of Molecular Physiology, Otto-Hahn-Straße 11, 44227 Dortmund, Germany.
Faesen AC; Department of Mechanistic Cell Biology, Max Planck Institute of Molecular Physiology, Otto-Hahn-Straße 11, 44227 Dortmund, Germany.
Petrovic A; Department of Mechanistic Cell Biology, Max Planck Institute of Molecular Physiology, Otto-Hahn-Straße 11, 44227 Dortmund, Germany.
Erent M; Centre for Mechanochemical Cell Biology, Warwick Medical School, The University of Warwick, Coventry, CV4 7AL, UK.
Maffini S; Department of Mechanistic Cell Biology, Max Planck Institute of Molecular Physiology, Otto-Hahn-Straße 11, 44227 Dortmund, Germany.
Pentakota S; Department of Mechanistic Cell Biology, Max Planck Institute of Molecular Physiology, Otto-Hahn-Straße 11, 44227 Dortmund, Germany.
Weir JR; Department of Mechanistic Cell Biology, Max Planck Institute of Molecular Physiology, Otto-Hahn-Straße 11, 44227 Dortmund, Germany.
Lin YC; Department of Mechanistic Cell Biology, Max Planck Institute of Molecular Physiology, Otto-Hahn-Straße 11, 44227 Dortmund, Germany.
Raunser S; Department of Structural Biochemistry, Max Planck Institute of Molecular Physiology, Otto-Hahn-Straße 11, 44227 Dortmund, Germany. Electronic address: stefan.raunser@mpi-dortmund.mpg.de.
McAinsh AD; Centre for Mechanochemical Cell Biology, Warwick Medical School, The University of Warwick, Coventry, CV4 7AL, UK. Electronic address: a.d.mcainsh@warwick.ac.uk.
Musacchio A; Department of Mechanistic Cell Biology, Max Planck Institute of Molecular Physiology, Otto-Hahn-Straße 11, 44227 Dortmund, Germany; Centre for Medical Biotechnology, Faculty of Biology, University Duisburg-Essen, Universitätsstraße, 45141 Essen, Germany. Electronic address: andrea.musacchio@mpi-dort

Mol Cell ; 71(6): 923-939.e10, 2018 09 20.

Article in En | MEDLINE | ID: mdl-30174292

Subject(s)

Chromosomal Proteins, Non-Histone/ultrastructure; Kinetochores/physiology; Kinetochores/ultrastructure; Centromere/physiology; Centromere Protein A/metabolism; Centromere Protein A/ultrastructure; Chromosomal Proteins, Non-Histone/metabolism; Cytoskeletal Proteins; HeLa Cells; Humans; Kinetochores/metabolism; Microtubules/metabolism; Microtubules/physiology; Nuclear Proteins/metabolism

Key words

CCAN; CENP-O; CENP-P; CENP-R; CENP-U; KMN network; centromere; constitutive centromere associated network; kinetochore; microtubule

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Chromosomal Proteins, Non-Histone / Kinetochores Limits: Humans Language: En Journal: Mol Cell Journal subject: BIOLOGIA MOLECULAR Year: 2018 Type: Article Affiliation country: Germany

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