Genomic profiles of primary and metastatic esophageal adenocarcinoma identified via digital sorting of pure cell populations: results from a case report.
BMC Cancer
; 18(1): 889, 2018 Sep 12.
Article
in En
| MEDLINE
| ID: mdl-30208867
ABSTRACT
BACKGROUND:
We report on a female patient who underwent primary radical resection for a stage 2B Her-2-positive Barrett's-type esophageal adenocarcinoma (EAC). Despite Her-2 targeted therapy, her disease recurred and required repeated metastectomies. CASE PRESENTATION Digital cell sorting and targeted sequencing of cancer sub-clones from EAC and metastases revealed a completely mutated TP53, whereas the sorted stromal cells were wild-type. Her-2 amplification was significantly lower in the metastases when the patient became therapy-resistant.CONCLUSIONS:
The mechanism of therapy resistance illustrated by this case could only be detected through accurate analysis of tumor sub-populations. Investigating tumor sub-populations of recurrent disease is important for adjusting therapy in recurrent EAC.Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Esophageal Neoplasms
/
Adenocarcinoma
/
Genomics
/
Clonal Evolution
/
Whole Genome Sequencing
Type of study:
Prognostic_studies
Limits:
Female
/
Humans
Language:
En
Journal:
BMC Cancer
Journal subject:
NEOPLASIAS
Year:
2018
Type:
Article
Affiliation country:
Italy