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Identification of the Human Skeletal Stem Cell.
Chan, Charles K F; Gulati, Gunsagar S; Sinha, Rahul; Tompkins, Justin Vincent; Lopez, Michael; Carter, Ava C; Ransom, Ryan C; Reinisch, Andreas; Wearda, Taylor; Murphy, Matthew; Brewer, Rachel E; Koepke, Lauren S; Marecic, Owen; Manjunath, Anoop; Seo, Eun Young; Leavitt, Tripp; Lu, Wan-Jin; Nguyen, Allison; Conley, Stephanie D; Salhotra, Ankit; Ambrosi, Thomas H; Borrelli, Mimi R; Siebel, Taylor; Chan, Karen; Schallmoser, Katharina; Seita, Jun; Sahoo, Debashis; Goodnough, Henry; Bishop, Julius; Gardner, Michael; Majeti, Ravindra; Wan, Derrick C; Goodman, Stuart; Weissman, Irving L; Chang, Howard Y; Longaker, Michael T.
Affiliation
  • Chan CKF; Department of Surgery, Stanford Medicine, Stanford, CA 94305, USA; Institute for Stem Cell Biology and Regenerative Medicine, Stanford Medicine, Stanford, CA 94305, USA. Electronic address: chazchan@stanford.edu.
  • Gulati GS; Department of Surgery, Stanford Medicine, Stanford, CA 94305, USA; Institute for Stem Cell Biology and Regenerative Medicine, Stanford Medicine, Stanford, CA 94305, USA.
  • Sinha R; Institute for Stem Cell Biology and Regenerative Medicine, Stanford Medicine, Stanford, CA 94305, USA.
  • Tompkins JV; Department of Surgery, Stanford Medicine, Stanford, CA 94305, USA.
  • Lopez M; Department of Surgery, Stanford Medicine, Stanford, CA 94305, USA.
  • Carter AC; Center for Personal Dynamic Regulomes, Stanford University, Stanford, CA 94305, USA.
  • Ransom RC; Department of Surgery, Stanford Medicine, Stanford, CA 94305, USA; Institute for Stem Cell Biology and Regenerative Medicine, Stanford Medicine, Stanford, CA 94305, USA.
  • Reinisch A; Institute for Stem Cell Biology and Regenerative Medicine, Stanford Medicine, Stanford, CA 94305, USA.
  • Wearda T; Department of Surgery, Stanford Medicine, Stanford, CA 94305, USA.
  • Murphy M; Department of Surgery, Stanford Medicine, Stanford, CA 94305, USA.
  • Brewer RE; Department of Surgery, Stanford Medicine, Stanford, CA 94305, USA.
  • Koepke LS; Department of Surgery, Stanford Medicine, Stanford, CA 94305, USA.
  • Marecic O; Department of Surgery, Stanford Medicine, Stanford, CA 94305, USA.
  • Manjunath A; Department of Surgery, Stanford Medicine, Stanford, CA 94305, USA; Institute for Stem Cell Biology and Regenerative Medicine, Stanford Medicine, Stanford, CA 94305, USA.
  • Seo EY; Department of Surgery, Stanford Medicine, Stanford, CA 94305, USA.
  • Leavitt T; Department of Surgery, Stanford Medicine, Stanford, CA 94305, USA.
  • Lu WJ; Institute for Stem Cell Biology and Regenerative Medicine, Stanford Medicine, Stanford, CA 94305, USA.
  • Nguyen A; Institute for Stem Cell Biology and Regenerative Medicine, Stanford Medicine, Stanford, CA 94305, USA.
  • Conley SD; Institute for Stem Cell Biology and Regenerative Medicine, Stanford Medicine, Stanford, CA 94305, USA.
  • Salhotra A; Department of Surgery, Stanford Medicine, Stanford, CA 94305, USA.
  • Ambrosi TH; Department of Surgery, Stanford Medicine, Stanford, CA 94305, USA.
  • Borrelli MR; Department of Surgery, Stanford Medicine, Stanford, CA 94305, USA.
  • Siebel T; Institute for Stem Cell Biology and Regenerative Medicine, Stanford Medicine, Stanford, CA 94305, USA.
  • Chan K; Institute for Stem Cell Biology and Regenerative Medicine, Stanford Medicine, Stanford, CA 94305, USA.
  • Schallmoser K; Department of Blood Group Serology and Transfusion Medicine, Medical University of Graz, 8036 Graz, Austria.
  • Seita J; Medical Sciences Innovation Hub Program, RIKEN, Tokyo 103-0027, Japan.
  • Sahoo D; Departments of Pediatrics and Computer Science and Engineering, University of California San Diego, San Diego, CA 92093, USA.
  • Goodnough H; Department of Orthopedic Surgery, Stanford Medicine, Stanford, CA 94305, USA.
  • Bishop J; Department of Orthopedic Surgery, Stanford Medicine, Stanford, CA 94305, USA.
  • Gardner M; Department of Orthopedic Surgery, Stanford Medicine, Stanford, CA 94305, USA.
  • Majeti R; Department of Medicine, Division of Hematology, Stanford Medicine, Stanford, CA 94305, USA.
  • Wan DC; Department of Surgery, Stanford Medicine, Stanford, CA 94305, USA.
  • Goodman S; Department of Orthopedic Surgery, Stanford Medicine, Stanford, CA 94305, USA.
  • Weissman IL; Institute for Stem Cell Biology and Regenerative Medicine, Stanford Medicine, Stanford, CA 94305, USA.
  • Chang HY; Center for Personal Dynamic Regulomes, Stanford University, Stanford, CA 94305, USA.
  • Longaker MT; Department of Surgery, Stanford Medicine, Stanford, CA 94305, USA; Institute for Stem Cell Biology and Regenerative Medicine, Stanford Medicine, Stanford, CA 94305, USA. Electronic address: longaker@stanford.edu.
Cell ; 175(1): 43-56.e21, 2018 09 20.
Article in En | MEDLINE | ID: mdl-30241615
ABSTRACT
Stem cell regulation and hierarchical organization of human skeletal progenitors remain largely unexplored. Here, we report the isolation of a self-renewing and multipotent human skeletal stem cell (hSSC) that generates progenitors of bone, cartilage, and stroma, but not fat. Self-renewing and multipotent hSSCs are present in fetal and adult bones and can also be derived from BMP2-treated human adipose stroma (B-HAS) and induced pluripotent stem cells (iPSCs). Gene expression analysis of individual hSSCs reveals overall similarity between hSSCs obtained from different sources and partially explains skewed differentiation toward cartilage in fetal and iPSC-derived hSSCs. hSSCs undergo local expansion in response to acute skeletal injury. In addition, hSSC-derived stroma can maintain human hematopoietic stem cells (hHSCs) in serum-free culture conditions. Finally, we combine gene expression and epigenetic data of mouse skeletal stem cells (mSSCs) and hSSCs to identify evolutionarily conserved and divergent pathways driving SSC-mediated skeletogenesis. VIDEO ABSTRACT.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Bone and Bones / Bone Development / Hematopoietic Stem Cells Type of study: Diagnostic_studies Limits: Animals / Humans Language: En Journal: Cell Year: 2018 Type: Article
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Bone and Bones / Bone Development / Hematopoietic Stem Cells Type of study: Diagnostic_studies Limits: Animals / Humans Language: En Journal: Cell Year: 2018 Type: Article