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Spatial Reconstruction of Single Enterocytes Uncovers Broad Zonation along the Intestinal Villus Axis.
Moor, Andreas E; Harnik, Yotam; Ben-Moshe, Shani; Massasa, Efi E; Rozenberg, Milena; Eilam, Raya; Bahar Halpern, Keren; Itzkovitz, Shalev.
Affiliation
  • Moor AE; Department of Molecular Cell Biology, Weizmann Institute of Science, Rehovot, Israel. Electronic address: andreas.moor@weizmann.ac.il.
  • Harnik Y; Department of Molecular Cell Biology, Weizmann Institute of Science, Rehovot, Israel.
  • Ben-Moshe S; Department of Molecular Cell Biology, Weizmann Institute of Science, Rehovot, Israel.
  • Massasa EE; Department of Molecular Cell Biology, Weizmann Institute of Science, Rehovot, Israel.
  • Rozenberg M; Department of Molecular Cell Biology, Weizmann Institute of Science, Rehovot, Israel.
  • Eilam R; Department of Veterinary Resources, Weizmann Institute of Science, Rehovot, Israel.
  • Bahar Halpern K; Department of Molecular Cell Biology, Weizmann Institute of Science, Rehovot, Israel.
  • Itzkovitz S; Department of Molecular Cell Biology, Weizmann Institute of Science, Rehovot, Israel. Electronic address: shalev.itzkovitz@weizmann.ac.il.
Cell ; 175(4): 1156-1167.e15, 2018 11 01.
Article in En | MEDLINE | ID: mdl-30270040
The intestinal epithelium is a highly structured tissue composed of repeating crypt-villus units. Enterocytes perform the diverse tasks of absorbing a wide range of nutrients while protecting the body from the harsh bacterium-rich environment. It is unknown whether these tasks are spatially zonated along the villus axis. Here, we extracted a large panel of landmark genes characterized by transcriptomics of laser capture microdissected villus segments and utilized it for single-cell spatial reconstruction, uncovering broad zonation of enterocyte function along the villus. We found that enterocytes at villus bottoms express an anti-bacterial gene program in a microbiome-dependent manner. They next shift to sequential expression of carbohydrates, peptides, and fat absorption machineries in distinct villus compartments. Finally, they induce a Cd73 immune-modulatory program at the villus tips. Our approach can be used to uncover zonation patterns in other organs when prior knowledge of landmark genes is lacking.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Enterocytes / Transcriptome Limits: Animals Language: En Journal: Cell Year: 2018 Type: Article

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Enterocytes / Transcriptome Limits: Animals Language: En Journal: Cell Year: 2018 Type: Article