PIE-1 Translation in the Germline Lineage Contributes to PIE-1 Asymmetry in the Early Caenorhabditis elegans Embryo.
G3 (Bethesda)
; 8(12): 3791-3801, 2018 12 10.
Article
in En
| MEDLINE
| ID: mdl-30279189
ABSTRACT
In the C. elegans embryo, the germline lineage is established through successive asymmetric cell divisions that each generate a somatic and a germline daughter cell. PIE-1 is an essential maternal factor that is enriched in embryonic germline cells and is required for germline specification. We estimated the absolute concentration of PIE-1GFP in germline cells and find that PIE-1GFP concentration increases by roughly 4.5 fold, from 92 nM to 424 nM, between the 1 and 4-cell stages. Previous studies have shown that the preferential inheritance of PIE-1 by germline daughter cells and the degradation of PIE-1 in somatic cells are important for PIE-1 enrichment in germline cells. In this study, we provide evidence that the preferential translation of maternal PIE-1GFP transcripts in the germline also contributes to PIE-1GFP enrichment. Through an RNAi screen, we identified Y14 and MAG-1 (Drosophila tsunagi and mago nashi) as regulators of embryonic PIE-1GFP levels. We show that Y14 and MAG-1 do not regulate PIE-1 degradation, segregation or synthesis in the early embryo, but do regulate the concentration of maternally-deposited PIE-1GFP. Taken together, or findings point to an important role for translational control in the regulation of PIE-1 levels in the germline lineage.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Protein Biosynthesis
/
Nuclear Proteins
/
Caenorhabditis elegans
/
Cell Lineage
/
Caenorhabditis elegans Proteins
/
Embryo, Nonmammalian
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Germ Cells
Type of study:
Prognostic_studies
Limits:
Animals
Language:
En
Journal:
G3 (Bethesda)
Year:
2018
Type:
Article