Enhanced validation of antibodies for research applications.
Nat Commun
; 9(1): 4130, 2018 10 08.
Article
in En
| MEDLINE
| ID: mdl-30297845
ABSTRACT
There is a need for standardized validation methods for antibody specificity and selectivity. Recently, five alternative validation pillars were proposed to explore the specificity of research antibodies using methods with no need for prior knowledge about the protein target. Here, we show that these principles can be used in a streamlined manner for enhanced validation of research antibodies in Western blot applications. More than 6,000 antibodies were validated with at least one of these strategies involving orthogonal methods, genetic knockdown, recombinant expression, independent antibodies, and capture mass spectrometry analysis. The results show a path forward for efforts to validate antibodies in an application-specific manner suitable for both providers and users.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Validation Studies as Topic
/
High-Throughput Screening Assays
/
Antibodies
/
Antibody Specificity
Limits:
Animals
/
Humans
Language:
En
Journal:
Nat Commun
Journal subject:
BIOLOGIA
/
CIENCIA
Year:
2018
Type:
Article
Affiliation country:
Sweden