Pervasive read-through transcription of T-DNAs is frequent in tobacco BY-2 cells and can effectively induce silencing.

Cermák, Vojtech; Fischer, Lukás

Cermák, Vojtech; Fischer, Lukás.

Affiliation

Cermák V; Department of Experimental Plant Biology, Charles University, Faculty of Science, Vinicná 5, 128 44, Prague 2, Czech Republic.
Fischer L; Department of Experimental Plant Biology, Charles University, Faculty of Science, Vinicná 5, 128 44, Prague 2, Czech Republic. lukas.fischer@natur.cuni.cz.

BMC Plant Biol ; 18(1): 252, 2018 Oct 22.

Article in En | MEDLINE | ID: mdl-30348096

Subject(s)

Agrobacterium tumefaciens/genetics; Arabidopsis/genetics; DNA, Bacterial/genetics; Nicotiana/genetics; Transcription, Genetic; Cell Line; Genes, Reporter; Inverted Repeat Sequences/genetics; Plants, Genetically Modified; Promoter Regions, Genetic/genetics; RNA, Messenger/genetics

Key words

GFP; Inverted repeat; Promoterless; RNAi; Read-through transcription; T-DNA; Tobacco BY-2 cell line

Fulltext

XML

PubMed Links

Search on Google

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Nicotiana / Transcription, Genetic / DNA, Bacterial / Agrobacterium tumefaciens / Arabidopsis Language: En Journal: BMC Plant Biol Journal subject: BOTANICA Year: 2018 Type: Article Affiliation country: Czech Republic

Fulltext

XML

PubMed Links

Search on Google