MiR-542-3p controls hepatic stellate cell activation and fibrosis via targeting BMP-7.
J Cell Biochem
; 120(3): 4573-4581, 2019 03.
Article
in En
| MEDLINE
| ID: mdl-30368874
ABSTRACT
There has been an increasing number of studies about microRNAs as key regulators in the development of hepatic fibrosis. Here, we demonstrate that miR-542-3p can promote hepatic fibrosis by downregulating the expression of bone morphogenetic protein 7 (BMP-7), which is known to antagonize transforming growth factor ß1 (TGFß1)-mediated fibrogenesis effect. The expression of miR-542-3p is increased in activated hepatic stellate cells (HSCs). Downregulation of MiR-542-3p by antisense inhibitors can inhibit HSCs activation markers, including α-smooth muscle actin (α-SMA) and collagen as well as TGFß signaling pathways. MiR-542-3p was significantly upregulated in carbon tetrachloride (CCl4 )-induced hepatic fibrosis in mice, and downregulation of miR-542-3p by lentivirus could prevent the development of hepatic fibrosis. In addition, miR-542-3p can directly bind to the 3'-untranslated region of BMP-7 mRNA, indicating that its profibrotic effect appears to be caused by its inhibition of BMP-7. Our results suggest that downregulation of miR-542-3p prevents liver fibrosis both in vitro and in vivo, highlighting its potential as a novel biomarker or therapeutic target for hepatic fibrosis.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Down-Regulation
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MicroRNAs
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Hepatic Stellate Cells
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Bone Morphogenetic Protein 7
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Liver Cirrhosis
Limits:
Animals
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Humans
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Male
Language:
En
Journal:
J Cell Biochem
Year:
2019
Type:
Article
Affiliation country:
China