DNA barcoding of species of Bacopa coupled with high-resolution melting analysis.
Genome
; 61(12): 867-877, 2018 Dec.
Article
in En
| MEDLINE
| ID: mdl-30388379
ABSTRACT
In Thailand, there are three species of Bacopa, namely, B. monnieri, B. caroliniana, and B. floribunda. Among these species of Bacopa, B. monnieri is the only medicinal species, used for the treatment of cognitive impairment and improvement of cognitive abilities because of its bioactive constituents, bacoside A and B. However, because of the similar characteristics of these species, it is difficult to differentiate among related species, resulting in confusion during identification. For this reason, and to ensure therapeutic quality for consumers, authentication is important. In this study, the three abovementioned species of Bacopa were evaluated using barcoding coupled with high-resolution melting (Bar-HRM) analysis based on primers designed for the trnL-F sequences of the three species. The melting profiles of the trnL-F amplicons of B. caroliniana and B. floribunda were clearly different from the melting profile of the trnL-F amplicon from B. monnieri; thus, the species could be discriminated by Bar-HRM analysis. Bar-HRM was then used to authenticate commercial products in various forms. The melting curves of the six commercial samples indicated that all the tested products contained genuine B. monnieri species. This method provides an efficient and reliable authentication system for future commercial herbal products and offers a reference system for quality control.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Bacopa
Type of study:
Prognostic_studies
Country/Region as subject:
Asia
Language:
En
Journal:
Genome
Journal subject:
GENETICA
Year:
2018
Type:
Article
Affiliation country:
Thailand