Single blastomeres as a source of mouse embryonic stem cells: effect of genetic background, medium supplements, and signaling modulators on derivation efficiency.

Vila-Cejudo, Marta; Massafret, Ot; Santaló, Josep; Ibáñez, Elena

Vila-Cejudo, Marta; Massafret, Ot; Santaló, Josep; Ibáñez, Elena.

Affiliation

Vila-Cejudo M; Departament de Biologia Cel·lular, Fisiologia i Immunologia, Facultat de Biociències, Universitat Autònoma de Barcelona, Bellaterra, Spain.
Massafret O; Tissue Engineering Unit, Centre for Genomic Regulation, Barcelona, Spain.
Santaló J; Departament de Biologia Cel·lular, Fisiologia i Immunologia, Facultat de Biociències, Universitat Autònoma de Barcelona, Bellaterra, Spain.
Ibáñez E; Departament de Biologia Cel·lular, Fisiologia i Immunologia, Facultat de Biociències, Universitat Autònoma de Barcelona, Bellaterra, Spain.

J Assist Reprod Genet ; 36(1): 99-111, 2019 Jan.

Article in En | MEDLINE | ID: mdl-30430313

Subject(s)

Blastomeres/cytology; Culture Media/pharmacology; Embryo Culture Techniques/methods; Genetic Background; Mouse Embryonic Stem Cells/cytology; Animals; Blastomeres/drug effects; Blastomeres/metabolism; Cell Differentiation; Embryonic Development; Female; Male; Mice; Mice, Inbred C57BL; Mice, Inbred CBA; Mouse Embryonic Stem Cells/drug effects; Mouse Embryonic Stem Cells/metabolism; Signal Transduction

Key words

Blastomere; ESC culture; ESC derivation; Mouse embryonic stem cells

Fulltext

XML

PubMed Links

Search on Google

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Blastomeres / Culture Media / Embryo Culture Techniques / Mouse Embryonic Stem Cells / Genetic Background Limits: Animals Language: En Journal: J Assist Reprod Genet Journal subject: GENETICA / MEDICINA REPRODUTIVA Year: 2019 Type: Article Affiliation country: Spain

Fulltext

XML

PubMed Links

Search on Google