Dynamics of Cas10 Govern Discrimination between Self and Non-self in Type III CRISPR-Cas Immunity.

Wang, Ling; Mo, Charlie Y; Wasserman, Michael R; Rostøl, Jakob T; Marraffini, Luciano A; Liu, Shixin

Wang, Ling; Mo, Charlie Y; Wasserman, Michael R; Rostøl, Jakob T; Marraffini, Luciano A; Liu, Shixin.

Affiliation

Wang L; Laboratory of Nanoscale Biophysics and Biochemistry, The Rockefeller University, 1230 York Avenue, New York, NY 10065, USA.
Mo CY; Laboratory of Bacteriology, The Rockefeller University, 1230 York Avenue, New York, NY 10065, USA.
Wasserman MR; Laboratory of Nanoscale Biophysics and Biochemistry, The Rockefeller University, 1230 York Avenue, New York, NY 10065, USA.
Rostøl JT; Laboratory of Bacteriology, The Rockefeller University, 1230 York Avenue, New York, NY 10065, USA.
Marraffini LA; Laboratory of Bacteriology, The Rockefeller University, 1230 York Avenue, New York, NY 10065, USA.
Liu S; Laboratory of Nanoscale Biophysics and Biochemistry, The Rockefeller University, 1230 York Avenue, New York, NY 10065, USA. Electronic address: shixinliu@rockefeller.edu.

Mol Cell ; 73(2): 278-290.e4, 2019 01 17.

Article in En | MEDLINE | ID: mdl-30503774

Subject(s)

Autoimmunity; Bacterial Proteins/immunology; CRISPR-Associated Proteins/immunology; CRISPR-Cas Systems/immunology; Clustered Regularly Interspaced Short Palindromic Repeats/immunology; RNA, Bacterial/immunology; Self Tolerance; Bacterial Proteins/genetics; Bacterial Proteins/metabolism; CRISPR-Associated Proteins/genetics; CRISPR-Associated Proteins/metabolism; CRISPR-Cas Systems/genetics; Clustered Regularly Interspaced Short Palindromic Repeats/genetics; Escherichia coli/enzymology; Escherichia coli/genetics; Escherichia coli/immunology; Kinetics; Microscopy, Fluorescence; Mutation; Nucleic Acid Conformation; Protein Conformation; RNA, Bacterial/chemistry; RNA, Bacterial/genetics; RNA, Bacterial/metabolism; Signal Transduction; Single Molecule Imaging/methods; Staphylococcus aureus/enzymology; Staphylococcus aureus/genetics; Staphylococcus aureus/immunology; Staphylococcus epidermidis/enzymology; Staphylococcus epidermidis/genetics; Staphylococcus epidermidis/immunology; Structure-Activity Relationship

Key words

Cas10; adaptive immunity; conformational dynamics; crRNA tag; protospacer; self/non-self discrimination; single-molecule FRET; target specificity; type III CRISPR-Cas

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Bacterial Proteins / RNA, Bacterial / Autoimmunity / Self Tolerance / CRISPR-Associated Proteins / Clustered Regularly Interspaced Short Palindromic Repeats / CRISPR-Cas Systems Type of study: Prognostic_studies Language: En Journal: Mol Cell Journal subject: BIOLOGIA MOLECULAR Year: 2019 Type: Article Affiliation country: United States

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