Fluorimetric Detection of Candida albicans Using Cornstalk N-Carbon Quantum Dots Modified with Amphotericin B.
Bioconjug Chem
; 30(3): 966-973, 2019 03 20.
Article
in En
| MEDLINE
| ID: mdl-30793876
ABSTRACT
Due to the advanced fluorescence property of N-carbon quantum dots (N-CQDs), a new method to detect pathogenic fungi by newly synthesized cornstalk N-CQDs modified with water-soluble amphotericin B (N-CQDs@AmpB) was developed. Specifically, N-CQDs with blue fluorescence were initially synthesized according to a previous report and modified with amphotericin B on their surfaces. Subsequently, the as-prepared N-CQDs@AmpB was used to detect Candida albicans, exhibiting a linear range of 2.60 × 105 to 1.99 × 108 cfu/mL and a detection limit of 1124 cfu/mL. Compared with other common methods, the method largely shortened the detection time and enabled the process to be performed with minimal interference from complex samples such as beef sausage. The high cost of water-soluble amphotericin B may hamper the large-scale application of the new detection method using N-CQDs@AmpB. Thus, alcohol-soluble amphotericin B was used in subsequent experiments, confirming its potential to broaden avenues for the detection of fungi.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Candida albicans
/
Carbon
/
Amphotericin B
/
Quantum Dots
/
Fluorometry
/
Antifungal Agents
Type of study:
Diagnostic_studies
Limits:
Animals
Language:
En
Journal:
Bioconjug Chem
Journal subject:
BIOQUIMICA
Year:
2019
Type:
Article