Identification of fungal isolates by MALDI-TOF mass spectrometry in veterinary practice: validation of a web application.
J Vet Diagn Invest
; 31(3): 471-474, 2019 May.
Article
in En
| MEDLINE
| ID: mdl-30943879
ABSTRACT
Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) has emerged as a reliable method to identify fungal isolates. The success of this approach relies on the availability of exhaustive databases, but the latter were built with a focus on human pathogens. We assessed a large in-house database of reference spectra and a dedicated web application for their suitability for use in veterinary laboratories. A panel of 290 mold and yeast isolates representing 69 different fungal species was isolated from various animals (including pets, cattle, and zoo animals) and identified using both MALDI-TOF MS and conventional techniques. The performance of the 2 methods was compared, and identifications were confirmed by DNA sequencing. MALDI-TOF MS allowed distinction between some closely related species and achieved 89% correct identification at the species level. In comparison, only 60% of the isolates were correctly identified with conventional approaches. Using this online application, MALDI-TOF MS thus appears to be a relevant alternative for the identification of fungal isolates encountered by animal health professionals.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Veterinary Medicine
/
Cattle
/
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
/
Pets
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Multilocus Sequence Typing
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Fungi
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Animals, Zoo
Type of study:
Diagnostic_studies
Limits:
Animals
Language:
En
Journal:
J Vet Diagn Invest
Journal subject:
MEDICINA VETERINARIA
Year:
2019
Type:
Article