R-DeeP: Proteome-wide and Quantitative Identification of RNA-Dependent Proteins by Density Gradient Ultracentrifugation.

Caudron-Herger, Maiwen; Rusin, Scott F; Adamo, Mark E; Seiler, Jeanette; Schmid, Vera K; Barreau, Elsa; Kettenbach, Arminja N; Diederichs, Sven

Caudron-Herger, Maiwen; Rusin, Scott F; Adamo, Mark E; Seiler, Jeanette; Schmid, Vera K; Barreau, Elsa; Kettenbach, Arminja N; Diederichs, Sven.

Affiliation

Caudron-Herger M; Division of RNA Biology and Cancer, German Cancer Research Center (DKFZ), 69120 Heidelberg, Germany. Electronic address: m.caudron@dkfz.de.
Rusin SF; Department of Biochemistry and Cell Biology, Geisel School of Medicine at Dartmouth, Hanover, NH 03755, USA.
Adamo ME; Norris Cotton Cancer Center, Geisel School of Medicine at Dartmouth, Lebanon, NH 03756, USA.
Seiler J; Division of RNA Biology and Cancer, German Cancer Research Center (DKFZ), 69120 Heidelberg, Germany.
Schmid VK; Division of RNA Biology and Cancer, German Cancer Research Center (DKFZ), 69120 Heidelberg, Germany.
Barreau E; Division of RNA Biology and Cancer, German Cancer Research Center (DKFZ), 69120 Heidelberg, Germany.
Kettenbach AN; Department of Biochemistry and Cell Biology, Geisel School of Medicine at Dartmouth, Hanover, NH 03755, USA; Norris Cotton Cancer Center, Geisel School of Medicine at Dartmouth, Lebanon, NH 03756, USA.
Diederichs S; Division of RNA Biology and Cancer, German Cancer Research Center (DKFZ), 69120 Heidelberg, Germany; Division of Cancer Research, Department of Thoracic Surgery, Medical Center-University of Freiburg, Faculty of Medicine, University of Freiburg, German Cancer Consortium (DKTK)- Partner Site Freiburg

Mol Cell ; 75(1): 184-199.e10, 2019 07 11.

Article in En | MEDLINE | ID: mdl-31076284

Subject(s)

Centrifugation, Density Gradient/methods; Protein Interaction Maps; Proteome/genetics; RNA-Binding Proteins/genetics; RNA/genetics; Transcription Factors/genetics; Centrifugation, Density Gradient/instrumentation; Chromatin/chemistry; Chromatin/metabolism; Gene Expression Regulation; Gene Ontology; HeLa Cells; Humans; Information Dissemination; Internet; Molecular Sequence Annotation; Protein Binding; Proteome/classification; Proteome/metabolism; Proteomics/methods; RNA/metabolism; RNA-Binding Proteins/classification; RNA-Binding Proteins/metabolism; Transcription Factors/classification; Transcription Factors/metabolism

Key words

CTCF; R-DeeP; RNA; RNA dependence; RNA-binding protein; RNase; density gradient; mass spectrometry; proteome-wide; proteomics

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Transcription Factors / RNA / Centrifugation, Density Gradient / RNA-Binding Proteins / Proteome / Protein Interaction Maps Type of study: Diagnostic_studies / Prognostic_studies Limits: Humans Language: En Journal: Mol Cell Journal subject: BIOLOGIA MOLECULAR Year: 2019 Type: Article

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