An improved nucleic acid extraction method from dried blood spots for amplification of Plasmodium falciparum kelch13 for detection of artemisinin resistance.

Zainabadi, Kayvan; Nyunt, Myaing M; Plowe, Christopher V

Zainabadi, Kayvan; Nyunt, Myaing M; Plowe, Christopher V.

Affiliation

Zainabadi K; Institute for Global Health, University of Maryland School of Medicine, Baltimore, MD, USA. kayvan@alum.mit.edu.
Nyunt MM; Center for Global Health, Weill Cornell Medicine, New York, NY, USA. kayvan@alum.mit.edu.
Plowe CV; Duke Global Health Institute, Duke University, Durham, NC, USA.

Malar J ; 18(1): 192, 2019 Jun 11.

Article in En | MEDLINE | ID: mdl-31185976

Subject(s)

Artemisinins/pharmacology; Blood/parasitology; DNA, Protozoan/isolation & purification; Drug Resistance; Malaria, Falciparum/parasitology; Plasmodium falciparum/genetics; Protozoan Proteins/genetics; Antimalarials/pharmacology; Asia, Southeastern; DNA, Protozoan/chemistry; DNA, Protozoan/genetics; Desiccation/methods; Humans; Plasmodium falciparum/drug effects; Polymerase Chain Reaction; Sequence Analysis, DNA; Specimen Handling/methods

Key words

Artemisinin resistance; Asymptomatic infection; DBS; Diagnostics; Dried blood spot; Drug resistance; K13; Kelch13; Low parasitaemia; Low transmission; Malaria; Molecular surveillance; PCR; Plasmodium falciparum; Southeast Asia

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Plasmodium falciparum / Blood / Drug Resistance / Protozoan Proteins / DNA, Protozoan / Malaria, Falciparum / Artemisinins Type of study: Diagnostic_studies / Guideline Limits: Humans Country/Region as subject: Asia Language: En Journal: Malar J Journal subject: MEDICINA TROPICAL Year: 2019 Type: Article Affiliation country: United States

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