[Construction of real-time polymerase chain reaction detection for infection-related cytokines of tree shrew].
Sheng Wu Yi Xue Gong Cheng Xue Za Zhi
; 36(3): 407-413, 2019 Jun 25.
Article
in Zh
| MEDLINE
| ID: mdl-31232543
ABSTRACT
Tree shrew is a novel and high-quality experimental animal model. In this study, the real-time polymerase chain reaction methods were established to detect infection-related cytokines interleukin-6 (IL-6), IL-8, IL-10, IL-17A, interferon-γ (IFN-γ) and housekeeping gene glyceraldehyde-phosphate dehydrogenase ( GAPDH) of tree shrew. The results indicated that the establised methods had good specificity. The high point of the linear range of these reagents reached 1 × 10 10 copies, and the low points ranged from 10 copies (IL-6, IL-17A), 100 copies (IL-10, GAPDH) to 1 000 copies (IL-8, IFN-γ). In this interval, the linear correlation coefficient R 2 of each reagent was greater than 0.99. The lowest detectable values of IL-6, IL-8, IL-10, IL-17A, IFN-γ and GAPDH were 8, 8, 4, 8, 128 and 4 copies, respectively. The results showed that the established detection methods had good specificity, sensitivity and wide linear range. The methods were suitable for detection of multiple concentration range samples, and could be used for the subsequent studies of tree shrew cytokines.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Shrews
/
Cytokines
/
Real-Time Polymerase Chain Reaction
Type of study:
Diagnostic_studies
Limits:
Animals
Language:
Zh
Journal:
Sheng Wu Yi Xue Gong Cheng Xue Za Zhi
Journal subject:
ENGENHARIA BIOMEDICA
Year:
2019
Type:
Article