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High-definition spatial transcriptomics for in situ tissue profiling.
Vickovic, Sanja; Eraslan, Gökcen; Salmén, Fredrik; Klughammer, Johanna; Stenbeck, Linnea; Schapiro, Denis; Äijö, Tarmo; Bonneau, Richard; Bergenstråhle, Ludvig; Navarro, José Fernandéz; Gould, Joshua; Griffin, Gabriel K; Borg, Åke; Ronaghi, Mostafa; Frisén, Jonas; Lundeberg, Joakim; Regev, Aviv; Ståhl, Patrik L.
Affiliation
  • Vickovic S; Klarman Cell Observatory, Broad Institute of MIT and Harvard, Cambridge, MA, USA. vickovic@broadinstitute.org.
  • Eraslan G; Science for Life Laboratory, Department of Gene Technology, KTH Royal Institute of Technology, Stockholm, Sweden. vickovic@broadinstitute.org.
  • Salmén F; Klarman Cell Observatory, Broad Institute of MIT and Harvard, Cambridge, MA, USA.
  • Klughammer J; Science for Life Laboratory, Department of Gene Technology, KTH Royal Institute of Technology, Stockholm, Sweden.
  • Stenbeck L; Klarman Cell Observatory, Broad Institute of MIT and Harvard, Cambridge, MA, USA.
  • Schapiro D; Science for Life Laboratory, Department of Gene Technology, KTH Royal Institute of Technology, Stockholm, Sweden.
  • Äijö T; Klarman Cell Observatory, Broad Institute of MIT and Harvard, Cambridge, MA, USA.
  • Bonneau R; Laboratory of Systems Pharmacology, Harvard Medical School, Boston, MA, USA.
  • Bergenstråhle L; Center for Computational Biology, Flatiron Institute, New York, NY, USA.
  • Navarro JF; Center for Data Science, New York University, New York, NY, USA.
  • Gould J; Department of Pathology, Brigham and Women's Hospital, Boston, MA, USA.
  • Griffin GK; Science for Life Laboratory, Department of Gene Technology, KTH Royal Institute of Technology, Stockholm, Sweden.
  • Borg Å; Science for Life Laboratory, Department of Gene Technology, KTH Royal Institute of Technology, Stockholm, Sweden.
  • Ronaghi M; Klarman Cell Observatory, Broad Institute of MIT and Harvard, Cambridge, MA, USA.
  • Frisén J; Klarman Cell Observatory, Broad Institute of MIT and Harvard, Cambridge, MA, USA.
  • Lundeberg J; Department of Pathology, Brigham and Women's Hospital, Boston, MA, USA.
  • Regev A; Division of Oncology and Pathology, Department of Clinical Sciences Lund, Lund University, Lund, Sweden.
  • Ståhl PL; Illumina, Inc., San Diego, CA, USA.
Nat Methods ; 16(10): 987-990, 2019 10.
Article in En | MEDLINE | ID: mdl-31501547
ABSTRACT
Spatial and molecular characteristics determine tissue function, yet high-resolution methods to capture both concurrently are lacking. Here, we developed high-definition spatial transcriptomics, which captures RNA from histological tissue sections on a dense, spatially barcoded bead array. Each experiment recovers several hundred thousand transcript-coupled spatial barcodes at 2-µm resolution, as demonstrated in mouse brain and primary breast cancer. This opens the way to high-resolution spatial analysis of cells and tissues.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Gene Expression Profiling / Transcriptome Limits: Animals / Female / Humans Language: En Journal: Nat Methods Journal subject: TECNICAS E PROCEDIMENTOS DE LABORATORIO Year: 2019 Type: Article Affiliation country: United States
Fulltext
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Gene Expression Profiling / Transcriptome Limits: Animals / Female / Humans Language: En Journal: Nat Methods Journal subject: TECNICAS E PROCEDIMENTOS DE LABORATORIO Year: 2019 Type: Article Affiliation country: United States