Efficient production of wild-type lipase B from Candida antarctica in the cytoplasm of Escherichia coli.

Van Tassel, Lisette; Moilanen, Antti; Ruddock, Lloyd W

Van Tassel, Lisette; Moilanen, Antti; Ruddock, Lloyd W.

Affiliation

Van Tassel L; Faculty of Biochemistry and Molecular Medicine and Biocenter Oulu, University of Oulu, Aapistie 7, 90220, Oulu, Finland.
Moilanen A; Faculty of Biochemistry and Molecular Medicine and Biocenter Oulu, University of Oulu, Aapistie 7, 90220, Oulu, Finland.
Ruddock LW; Faculty of Biochemistry and Molecular Medicine and Biocenter Oulu, University of Oulu, Aapistie 7, 90220, Oulu, Finland. Electronic address: Lloyd.ruddock@oulu.fi.

Protein Expr Purif ; 165: 105498, 2020 01.

Article in En | MEDLINE | ID: mdl-31521797

Subject(s)

Cytoplasm/metabolism; Escherichia coli/metabolism; Fungal Proteins/genetics; Fungal Proteins/isolation & purification; Lipase/genetics; Lipase/isolation & purification; Base Sequence; Catalysis; Cloning, Molecular; Disulfides/metabolism; Escherichia coli/ultrastructure; Fermentation; Fungal Proteins/metabolism; Gene Expression Regulation, Bacterial; Lipase/metabolism; Oxidation-Reduction; Protein Conformation; Signal Transduction; Transfection

Key words

(max 6) Escherichia coli; CalB; Cytoplasmic oxidative folding; Disulfide bond formation; Lipase

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Fungal Proteins / Cytoplasm / Escherichia coli / Lipase Language: En Journal: Protein Expr Purif Journal subject: BIOLOGIA MOLECULAR Year: 2020 Type: Article Affiliation country: Finland

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