A novel differential scanning fluorimetry analysis of a humanized anti-cocaine mAb and its ligand binding characteristics.

Kirley, Terence L; Norman, Andrew B; Wetzel, Hanna N

Kirley, Terence L; Norman, Andrew B; Wetzel, Hanna N.

Affiliation

Kirley TL; From the Department of Pharmacology and Systems Physiology, College of Medicine, University of Cincinnati, 231 Albert Sabin Way, Cincinnati, OH 45267-0575, United States of America. Electronic address: terry.kirley@uc.edu.
Norman AB; From the Department of Pharmacology and Systems Physiology, College of Medicine, University of Cincinnati, 231 Albert Sabin Way, Cincinnati, OH 45267-0575, United States of America.
Wetzel HN; From the Department of Pharmacology and Systems Physiology, College of Medicine, University of Cincinnati, 231 Albert Sabin Way, Cincinnati, OH 45267-0575, United States of America.

J Immunol Methods ; 476: 112676, 2020 01.

Article in En | MEDLINE | ID: mdl-31634480

Subject(s)

Antibodies, Monoclonal, Humanized/immunology; Cocaine/immunology; Fluorescent Dyes; Fluorometry/methods; Pyridinium Compounds; Antigen-Antibody Reactions; Binding Sites, Antibody; Cocaine/analogs & derivatives; Humans; Immunoglobulin Fab Fragments/immunology; Ligands

Key words

DASPMI rotor dye; Differential scanning fluorimetry; Ligand binding; Monoclonal antibody; Protein denaturation; Thermodynamics

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Pyridinium Compounds / Cocaine / Antibodies, Monoclonal, Humanized / Fluorescent Dyes / Fluorometry Limits: Humans Language: En Journal: J Immunol Methods Year: 2020 Type: Article

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