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An atypical HLH transcriptional regulator plays a novel and important role in strawberry ripened receptacle.
Medina-Puche, Laura; Martínez-Rivas, Félix J; Molina-Hidalgo, Francisco J; Mercado, José A; Moyano, Enriqueta; Rodríguez-Franco, Antonio; Caballero, José L; Muñoz-Blanco, Juan; Blanco-Portales, Rosario.
Affiliation
  • Medina-Puche L; Departamento de Bioquímica y Biología Molecular, Edificio Severo Ochoa C-6, Campus Universitario de Rabanales y Campus de Excelencia Internacional Agroalimentario CEIA3, Universidad de Córdoba, Córdoba, Spain.
  • Martínez-Rivas FJ; Present Address: Shanghai Center for Plant Stress Biology (PSC), Shanghai Institutes of Biological Sciences, Chinese Academy of Sciences, Shanghai, China.
  • Molina-Hidalgo FJ; Departamento de Bioquímica y Biología Molecular, Edificio Severo Ochoa C-6, Campus Universitario de Rabanales y Campus de Excelencia Internacional Agroalimentario CEIA3, Universidad de Córdoba, Córdoba, Spain.
  • Mercado JA; Departamento de Bioquímica y Biología Molecular, Edificio Severo Ochoa C-6, Campus Universitario de Rabanales y Campus de Excelencia Internacional Agroalimentario CEIA3, Universidad de Córdoba, Córdoba, Spain.
  • Moyano E; Present Address: VIB-UGent Center for Plant Systems Biology, Ghent, Belgium.
  • Rodríguez-Franco A; Instituto de Hortofruticultura Subtropical y Mediterránea La Mayora (IHSM-UMA-CSIC), Departamento de Biología Vegetal, Universidad de Málaga, Málaga, Spain.
  • Caballero JL; Departamento de Bioquímica y Biología Molecular, Edificio Severo Ochoa C-6, Campus Universitario de Rabanales y Campus de Excelencia Internacional Agroalimentario CEIA3, Universidad de Córdoba, Córdoba, Spain.
  • Muñoz-Blanco J; Departamento de Bioquímica y Biología Molecular, Edificio Severo Ochoa C-6, Campus Universitario de Rabanales y Campus de Excelencia Internacional Agroalimentario CEIA3, Universidad de Córdoba, Córdoba, Spain.
  • Blanco-Portales R; Departamento de Bioquímica y Biología Molecular, Edificio Severo Ochoa C-6, Campus Universitario de Rabanales y Campus de Excelencia Internacional Agroalimentario CEIA3, Universidad de Córdoba, Córdoba, Spain.
BMC Plant Biol ; 19(1): 586, 2019 Dec 27.
Article in En | MEDLINE | ID: mdl-31881835
ABSTRACT

BACKGROUND:

In soft fruits, the differential expression of many genes during development and ripening is responsible for changing their organoleptic properties. In strawberry fruit, although some genes involved in the metabolic regulation of the ripening process have been functionally characterized, some of the most studied genes correspond to transcription factors. High throughput transcriptomics analyses performed in strawberry red receptacle (Fragaria x ananassa) allowed us to identify a ripening-related gene that codes an atypical HLH (FaPRE1) with high sequence homology with the PACLOBUTRAZOL RESISTANCE (PRE) genes. PRE genes are atypical bHLH proteins characterized by the lack of a DNA-binding domain and whose function has been linked to the regulation of cell elongation processes.

RESULTS:

FaPRE1 sequence analysis indicates that this gene belongs to the subfamily of atypical bHLHs that also includes ILI-1 from rice, SlPRE2 from tomato and AtPRE1 from Arabidopsis, which are involved in transcriptional regulatory processes as repressors, through the blockage by heterodimerization of bHLH transcription factors. FaPRE1 presented a transcriptional model characteristic of a ripening-related gene with receptacle-specific expression, being repressed by auxins and activated by abscisic acid (ABA). However, its expression was not affected by gibberellic acid (GA3). On the other hand, the transitory silencing of FaPRE1 transcription by agroinfiltration in receptacle produced the down-regulation of a group of genes related to the ripening process while inducing the transcription of genes involved in receptacle growth and development.

CONCLUSIONS:

In summary, this work presents for the first time experimental data that support an important novel function for the atypical HLH FaPRE1 during the strawberry fruit ripening. We hypothesize that FaPRE1 modulates antagonistically the transcription of genes related to both receptacle growth and ripening. Thus, FaPRE1 would repress the expression of receptacle growth promoting genes in the ripened receptacle, while it would activate the expression of those genes related to the receptacle ripening process.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Plant Proteins / Transcription Factors / Fragaria / Basic Helix-Loop-Helix Transcription Factors Type of study: Prognostic_studies Language: En Journal: BMC Plant Biol Journal subject: BOTANICA Year: 2019 Type: Article Affiliation country: Spain

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Plant Proteins / Transcription Factors / Fragaria / Basic Helix-Loop-Helix Transcription Factors Type of study: Prognostic_studies Language: En Journal: BMC Plant Biol Journal subject: BOTANICA Year: 2019 Type: Article Affiliation country: Spain