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Clinical Utility of Universal Broad-Range Polymerase Chain Reaction Amplicon Sequencing for Pathogen Identification: A Retrospective Cohort Study.
Kerkhoff, Andrew D; Rutishauser, Rachel L; Miller, Steve; Babik, Jennifer M.
Affiliation
  • Kerkhoff AD; Division of HIV, Infectious Diseases, and Global Medicine, Zuckerberg San Francisco General Hospital and Trauma Center, Department of Medicine, University of California, San Francisco, California, USA.
  • Rutishauser RL; Division of Experimental Medicine, Department of Medicine, University of California San Francisco, San Francisco, California, USA.
  • Miller S; Department of Laboratory Medicine, University of California, San Francisco, California, USA.
  • Babik JM; Division of Infectious Diseases, Department of Medicine, University of California, San Francisco, California, USA.
Clin Infect Dis ; 71(6): 1554-1557, 2020 09 12.
Article in En | MEDLINE | ID: mdl-31907545
We assessed the real-world utility of universal broad-range polymerase chain reaction sequencing for pathogen detection. Among 1062 clinical samples, 107/1062 (10.1%) had a clinically significant, positive result, with substantial variation by specimen type. Clinical management was changed in 44/1062 (4.1%). These data can help maximize utility of this emerging diagnostic.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Polymerase Chain Reaction Type of study: Diagnostic_studies / Etiology_studies / Observational_studies Limits: Humans Language: En Journal: Clin Infect Dis Journal subject: DOENCAS TRANSMISSIVEIS Year: 2020 Type: Article Affiliation country: United States
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Polymerase Chain Reaction Type of study: Diagnostic_studies / Etiology_studies / Observational_studies Limits: Humans Language: En Journal: Clin Infect Dis Journal subject: DOENCAS TRANSMISSIVEIS Year: 2020 Type: Article Affiliation country: United States