Your browser doesn't support javascript.
loading
LncRNA MCM3AP-AS1 inhibits cell proliferation in cervical squamous cell carcinoma by down-regulating miRNA-93.
Lan, Liu; Liang, Zhishan; Zhao, Yingxi; Mo, Yuzhen.
Affiliation
  • Lan L; Department of Radiotheragy, The Second Affiliated Hospital of Guangxi University of Science and Technology, Liuzhou, Guangxi 545005, P.R. China.
  • Liang Z; Department of Cardiology, Huadu District People's Hospital, Southern Medical University, Guangzhou, Guangdong 510800, P.R. China.
  • Zhao Y; Department of Radiotheragy, The Second Affiliated Hospital of Guangxi University of Science and Technology, Liuzhou, Guangxi 545005, P.R. China.
  • Mo Y; Department of Radiotheragy, Guangzhou Red Cross Hospital, Medical College, Jinan University, Guangzhou, Guangdong 510220, P.R. China.
Biosci Rep ; 40(2)2020 02 28.
Article in En | MEDLINE | ID: mdl-31985002
BACKGROUND: MCM3AP antisense RNA 1 (MCM3AP-AS1) is characterized as an oncogenic lncRNA in hepatocellular carcinoma and glioblastoma. We analyzed TCGA dataset and observed the down-regulation of MCM3AP-AS1 in cervical squamous cell carcinoma (CSCC). The present study was therefore performed to investigate the role of MCM3AP-AS1 in CSCC. METHODS: A total of 64 female patients with CSCC (38-68 years old; mean age: 53.1 ± 6.5 years old) were enrolled in the present study. RT-qPCR was performed to evaluate gene expression. Methylation specific PCR (MSP) was performed to assess the methylation of miR-93 gene after the overexpression and silencing of MCM3AP-AS1. Cell transfections were performed to investigate the interactions between MCM3AP-AS1 and miR-93. Cell proliferation was assessed by CCK-8 assay. RESULTS: The results showed that MCM3AP-AS1 was down-regulated in CSCC and predicted poor survival. The expression levels of MCM3AP-AS1 were inversely correlated with the expression levels of miR-93. Overexpression of MCM3AP-AS1 led to down-regulation of miR-93, while silencing of MCM3AP-AS1 played an opposite role in CSCC cells. Methylation-specific PCR revealed that MCM3AP-AS1 could positively regulate the methylation of miR-93 gene. Cell proliferation analysis showed that overexpression of MCM3AP-AS1 led to reduced proliferation rate of CSCC cells. Silencing of MCM3AP-AS1 played an opposite role and overexpression of miR-93 reduced the effects of overexpressing MCM3AP-AS1. CONCLUSIONS: Therefore, MCM3AP-AS1 may inhibit cell proliferation in CSCC by down-regulating miRNA-93.
Subject(s)
Key words

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Carcinoma, Squamous Cell / Uterine Cervical Neoplasms / MicroRNAs / Cell Proliferation / RNA, Long Noncoding Limits: Adult / Aged / Female / Humans / Middle aged Language: En Journal: Biosci Rep Year: 2020 Type: Article

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Carcinoma, Squamous Cell / Uterine Cervical Neoplasms / MicroRNAs / Cell Proliferation / RNA, Long Noncoding Limits: Adult / Aged / Female / Humans / Middle aged Language: En Journal: Biosci Rep Year: 2020 Type: Article