Your browser doesn't support javascript.
loading
NAD(P)-Driven Redox Status Contributes to Desiccation Tolerance in Acer seeds.
Alipour, Shirin; Wojciechowska, Natalia; Stolarska, Ewelina; Bilska, Karolina; Kalemba, Ewa Marzena.
Affiliation
  • Alipour S; Institute of Dendrology, Polish Academy of Sciences, Parkowa 5, 62-035 Kórnik, Poland.
  • Wojciechowska N; Department of Forestry, Faculty of Agriculture and Natural Resources, Lorestan University, Khorramabad, Iran.
  • Stolarska E; Institute of Dendrology, Polish Academy of Sciences, Parkowa 5, 62-035 Kórnik, Poland.
  • Bilska K; Department of General Botany, Institute of Experimental Biology, Faculty of Biology, Adam Mickiewicz University, Uniwersytetu Poznanskiego 6, 61-614 Poznan, Poland.
  • Kalemba EM; Institute of Dendrology, Polish Academy of Sciences, Parkowa 5, 62-035 Kórnik, Poland.
Plant Cell Physiol ; 61(6): 1158-1167, 2020 Jun 01.
Article in En | MEDLINE | ID: mdl-32267948
ABSTRACT
Desiccation tolerance is a developmental program enabling seed survival in a dry state and is common in seeds categorized as orthodox. We focused on NAD and its phosphorylated form (NADP) because their continual switching between reduced (NAD(P)H) and oxidized (NAD(P)+) forms is involved in the modulation of redox signaling and the determination of the reducing power and further antioxidant responses. Norway maple and sycamore seeds representing the orthodox and recalcitrant categories, respectively, were used as models in a comparison of responses to water loss. The process of desiccation up to 10% water content (WC) was monitored in Norway maple seeds, while dehydration up to 30% WC was monitored in desiccation-sensitive sycamore seeds. Norway maple and sycamore seeds, particularly their embryonic axes, exhibited a distinct redox status during dehydration and desiccation. High NADPH levels, NAD+ accumulation, low and stable NAD(P)H/NAD(P)+ ratios expressed as reducing power and high NADPH-dependent enzyme activity were reported in Norway maple seeds and were considered attributes of orthodox-type seeds. The contrasting results of sycamore seeds contributed to their low antioxidant capacity and high sensitivity to desiccation. NADPH deficiency, low NADPH-dependent enzyme activity and lack of NAD+ accumulation were primary features of sycamore seeds, with implications for their NAD(P)H/NAD(P)+ ratios and reducing power and with effects on many seed traits. Thus, we propose that the distinct levels of pyridine nucleotides and their redox status contribute to orthodox and recalcitrant phenotype differentiation in seeds by affecting cellular redox signaling, metabolism and the antioxidant system.
Subject(s)
Key words

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Oxidation-Reduction / Seeds / Acer / NADP Language: En Journal: Plant Cell Physiol Journal subject: BOTANICA Year: 2020 Type: Article Affiliation country: Poland

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Oxidation-Reduction / Seeds / Acer / NADP Language: En Journal: Plant Cell Physiol Journal subject: BOTANICA Year: 2020 Type: Article Affiliation country: Poland