A Circulating MicroRNA Profile in a Laser-Induced Mouse Model of Choroidal Neovascularization.
Int J Mol Sci
; 21(8)2020 Apr 13.
Article
in En
| MEDLINE
| ID: mdl-32294914
ABSTRACT
Choroidal neovascularization (CNV) is a pathological process in which aberrant blood vessels invade the subretinal space of the mammalian eye. It is a characteristic feature of the prevalent neovascular age-related macular degeneration (nAMD). Circulating microRNAs (cmiRNAs) are regarded as potentially valuable biomarkers for various age-related diseases, including nAMD. Here, we investigated cmiRNA expression in an established laser-induced CNV mouse model. Upon CNV induction in C57Bl/6 mice, blood-derived cmiRNAs were initially determined globally by RNA next generation sequencing, and the most strongly dysregulated cmiRNAs were independently replicated by quantitative reverse transcription PCR (RT-qPCR) in blood, retinal, and retinal pigment epithelium (RPE)/choroidal tissue. Our findings suggest that two miRNAs, mmu-mir-486a-5p and mmur-mir-92a-3p, are consistently dysregulated during CNV formation. Furthermore, in functional in vitro assays, a significant impact of mmu-mir-486a-5p and mmu-mir-92a-3p on murine microglial cell viability was observed, while mmu-mir-92a-3p also showed an impact on microglial mobility. Taken together, we report a robust dysregulation of two miRNAs in blood and RPE/choroid after laser-induced initiation of CNV lesions in mice, highlighting their potential role in pathology and eventual therapy of CNV-associated complications.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Choroidal Neovascularization
/
Circulating MicroRNA
/
Lasers
Type of study:
Prognostic_studies
/
Systematic_reviews
Limits:
Animals
Language:
En
Journal:
Int J Mol Sci
Year:
2020
Type:
Article
Affiliation country:
Germany