Relationship between Postmortem Interval and FTIR Spectroscopy Changes of the Rat Skin.
Fa Yi Xue Za Zhi
; 36(2): 187-191, 2020 Apr.
Article
in En, Zh
| MEDLINE
| ID: mdl-32530165
ABSTRACT
ABSTRACT Objective To infer postmortem interval ï¼PMIï¼ based on spectral changes of the dorsal skin of rats within 15 days postmortem using Fourier transform infrared ï¼FTIRï¼ spectroscopy. Methods The rats were sacrificed by cervical dislocation after anesthesia, and then placed at 25 â and relative humidity of 50%. The FTIR spectral data collected from the dorsal skin at PMI points were modeled with machine learning technique. Results There was no significant difference of absorption peak location among all the PMI groups but their peak intensities changed as a function of PMIs. The model for PMI estimation was constructed using partial least squares ï¼PLSï¼ regression, reaching a R2 of 0.92 and a root mean square error ï¼RMSEï¼ of 1.30 d. As shown in variable importance for projection ï¼VIPï¼, four spectral bands including 1 760-1 700 cm-1, 1 660-1 640 cm-1, 1 580-1 540 cm-1 and 1 460-1 420 cm-1 were determined as important contributions to model prediction. Conclusion Application of the FTIR technique to detect postmortem spectral changes of the rat skin provides a novel proposal for PMI estimation.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Postmortem Changes
Type of study:
Prognostic_studies
Limits:
Animals
Language:
En
/
Zh
Journal:
Fa Yi Xue Za Zhi
Journal subject:
JURISPRUDENCIA
Year:
2020
Type:
Article
Affiliation country:
China