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Visible Light Mediated Bidirectional Control over Carbonic Anhydrase Activity in Cells and in Vivo Using Azobenzenesulfonamides.
Aggarwal, Kanchan; Kuka, Timothy P; Banik, Mandira; Medellin, Brenda P; Ngo, Chinh Q; Xie, Da; Fernandes, Yohaan; Dangerfield, Tyler L; Ye, Elva; Bouley, Bailey; Johnson, Kenneth A; Zhang, Yan Jessie; Eberhart, Johann K; Que, Emily L.
Affiliation
  • Aggarwal K; Department of Chemistry, University of Texas at Austin, 105 E. 24th Street Stop A5300, Austin, Texas 78712, United States.
  • Kuka TP; Department of Molecular Biosciences and Institute for Cellular and Molecular Biology, University of Texas at Austin, 100 E. 24th Street Stop A5000, Austin, Texas 78712, United States.
  • Banik M; Department of Chemistry, University of Texas at Austin, 105 E. 24th Street Stop A5300, Austin, Texas 78712, United States.
  • Medellin BP; Department of Molecular Biosciences and Institute for Cellular and Molecular Biology, University of Texas at Austin, 100 E. 24th Street Stop A5000, Austin, Texas 78712, United States.
  • Ngo CQ; Department of Chemistry, University of Texas at Austin, 105 E. 24th Street Stop A5300, Austin, Texas 78712, United States.
  • Xie D; Department of Chemistry, University of Texas at Austin, 105 E. 24th Street Stop A5300, Austin, Texas 78712, United States.
  • Fernandes Y; Department of Molecular Biosciences and Institute for Cellular and Molecular Biology, University of Texas at Austin, 100 E. 24th Street Stop A5000, Austin, Texas 78712, United States.
  • Dangerfield TL; Waggoner Center for Alcohol and Addiction Research, The University of Texas at Austin, 2500 Speedway, A4800, Austin, Texas 78712, United States.
  • Ye E; Department of Molecular Biosciences and Institute for Cellular and Molecular Biology, University of Texas at Austin, 100 E. 24th Street Stop A5000, Austin, Texas 78712, United States.
  • Bouley B; Department of Chemistry, University of Texas at Austin, 105 E. 24th Street Stop A5300, Austin, Texas 78712, United States.
  • Johnson KA; Department of Chemistry, University of Texas at Austin, 105 E. 24th Street Stop A5300, Austin, Texas 78712, United States.
  • Zhang YJ; Department of Molecular Biosciences and Institute for Cellular and Molecular Biology, University of Texas at Austin, 100 E. 24th Street Stop A5000, Austin, Texas 78712, United States.
  • Eberhart JK; Department of Molecular Biosciences and Institute for Cellular and Molecular Biology, University of Texas at Austin, 100 E. 24th Street Stop A5000, Austin, Texas 78712, United States.
  • Que EL; Department of Molecular Biosciences and Institute for Cellular and Molecular Biology, University of Texas at Austin, 100 E. 24th Street Stop A5000, Austin, Texas 78712, United States.
J Am Chem Soc ; 142(34): 14522-14531, 2020 08 26.
Article in En | MEDLINE | ID: mdl-32623882
ABSTRACT
Two azobenzenesulfonamide molecules with thermally stable cis configurations resulting from fluorination of positions ortho to the azo group are reported that can differentially regulate the activity of carbonic anhydrase in the trans and cis configurations. These fluorinated probes each use two distinct visible wavelengths (520 and 410 or 460 nm) for isomerization with high photoconversion efficiency. Correspondingly, the cis isomer of these systems is highly stable and persistent (as evidenced by structural studies in solid and solution state), permitting regulation of metalloenzyme activity without continuous irradiation. Herein, we use these probes to demonstrate the visible light mediated bidirectional control over the activity of zinc-dependent carbonic anhydrase in solution as an isolated protein, in intact live cells and in vivo in zebrafish during embryo development.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Sulfonamides / Azo Compounds / Molecular Probes / Carbonic Anhydrases / Light Limits: Animals / Humans Language: En Journal: J Am Chem Soc Year: 2020 Type: Article Affiliation country: United States
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Sulfonamides / Azo Compounds / Molecular Probes / Carbonic Anhydrases / Light Limits: Animals / Humans Language: En Journal: J Am Chem Soc Year: 2020 Type: Article Affiliation country: United States