Simultaneous detection of three zoonotic pathogens based on phage display peptide and multicolor quantum dots.
Anal Biochem
; 608: 113854, 2020 11 01.
Article
in En
| MEDLINE
| ID: mdl-32745543
ABSTRACT
With the rapid development of human's exploitation of nature and animal husbandry, zoonoses have become a major public health problem worldwide. It is necessary to establish a rapid, specific and sensitive detection method to screen several zoonotic pathogenic bacteria simultaneously. In this study, phage display technology was used to screen specific peptide of three common zoonotic pathogens, E. coli O157H7, L. monocytogenes and B. melitensis 16 M. Then, three peptide were obtained, named E2, L4 and B4, which can identify the three pathogens respectively. Three peptide modified with biotin were synthesized and were coupled to streptavidin magnetic beads (MBs) to form peptide-MBs, which enriched the above three pathogens from the samples. Three quantum dot (QD) probes were constructed by coupling three polyclonal antibodies to different fluorescent QD surfaces (QD540, QD580 and QD630). The simultaneous detection method based on peptide-MBs and QDs multicolor fluorescent labeling was established and could detect E. coli O157H7, L. monocytogenes and B. melitensis 16 M simultaneously. The detection method took about 100 min with the detection limits of 103, 102 and 102 CFU/mL, respectively. The detection method could be also well utilized in real samples.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Peptides
/
Bacteria
/
Chemistry Techniques, Analytical
/
Immunomagnetic Separation
/
Colorimetry
/
Peptide Library
/
Quantum Dots
Type of study:
Diagnostic_studies
Limits:
Animals
Language:
En
Journal:
Anal Biochem
Year:
2020
Type:
Article
Affiliation country:
China