Viral Vectors for Neural Circuit Mapping and Recent Advances in Trans-synaptic Anterograde Tracers.
Neuron
; 107(6): 1029-1047, 2020 09 23.
Article
in En
| MEDLINE
| ID: mdl-32755550
ABSTRACT
Viral tracers are important tools for neuroanatomical mapping and genetic payload delivery. Genetically modified viruses allow for cell-type-specific targeting and overcome many limitations of non-viral tracers. Here, we summarize the viruses that have been developed for neural circuit mapping, and we provide a primer on currently applied anterograde and retrograde viral tracers with practical guidance on experimental uses. We also discuss and highlight key technical and conceptual considerations for developing new safer and more effective anterograde trans-synaptic viral vectors for neural circuit analysis in multiple species.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Synapses
/
Viruses
/
Neuroanatomical Tract-Tracing Techniques
/
Connectome
Type of study:
Guideline
Limits:
Animals
/
Humans
Language:
En
Journal:
Neuron
Journal subject:
NEUROLOGIA
Year:
2020
Type:
Article