Genetic engineering of sex chromosomes for batch cultivation of non-transgenic, sex-sorted males.
PLoS Genet
; 16(11): e1009180, 2020 11.
Article
in En
| MEDLINE
| ID: mdl-33137115
ABSTRACT
The field performance of Sterile Insect Technique (SIT) is improved by sex-sorting and releasing only sterile males. This can be accomplished by resource-intensive separation of males from females by morphology. Alternatively, sex-ratio biasing genetic constructs can be used to selectively remove one sex without the need for manual or automated sorting, but the resulting genetically engineered (GE) control agents would be subject to additional governmental regulation. Here we describe and demonstrate a genetic method for the batch production of non-GE males. This method could be applied to generate the heterogametic sex (XY, or WZ) in any organism with chromosomal sex determination. We observed up to 100% sex-selection with batch cultures of more than 103 individuals. Using a stringent transgene detection assay, we demonstrate the potential of mass production of transgene free males.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Sex Chromosomes
/
Genetic Engineering
/
Pest Control, Biological
/
Insect Control
Limits:
Animals
Language:
En
Journal:
PLoS Genet
Journal subject:
GENETICA
Year:
2020
Type:
Article
Affiliation country:
United States