Technical and Methodological Aspects of Cell-Free Nucleic Acids Analyzes.
Int J Mol Sci
; 21(22)2020 Nov 16.
Article
in En
| MEDLINE
| ID: mdl-33207777
ABSTRACT
Analyzes of cell-free nucleic acids (cfNAs) have shown huge potential in many biomedical applications, gradually entering several fields of research and everyday clinical care. Many biological properties of cfNAs can be informative to gain deeper insights into the function of the organism, such as their different types (DNA, RNAs) and subtypes (gDNA, mtDNA, bacterial DNA, miRNAs, etc.), forms (naked or vesicle bound NAs), fragmentation profiles, sequence composition, epigenetic modifications, and many others. On the other hand, the workflows of their analyzes comprise many important steps, from sample collection, storage and transportation, through extraction and laboratory analysis, up to bioinformatic analyzes and statistical evaluations, where each of these steps has the potential to affect the outcome and informational value of the performed analyzes. There are, however, no universal or standard protocols on how to exactly proceed when analyzing different cfNAs for different applications, at least according to our best knowledge. We decided therefore to prepare an overview of the available literature and products commercialized for cfNAs processing, in an attempt to summarize the benefits and limitations of the currently available approaches, devices, consumables, and protocols, together with various factors influencing the workflow, its processes, and outcomes.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
DNA, Bacterial
/
DNA, Mitochondrial
/
RNA, Bacterial
/
Cell-Free Nucleic Acids
/
RNA, Mitochondrial
Type of study:
Guideline
Language:
En
Journal:
Int J Mol Sci
Year:
2020
Type:
Article
Affiliation country:
Slovakia