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A multiplex PCR assay for the detection of five human pathogenic Vibrio species and Plesiomonas.
Guan, Hongxia; Xue, Panpan; Zhou, Haijian; Sha, Dan; Wang, Duochun; Gao, He; Li, Jie; Diao, Baowei; Zhao, Hongqun; Kan, Biao; Zhang, Jingyun.
Affiliation
  • Guan H; Wuxi Center for Disease Control and Prevention, Jiangsu, 214023, China.
  • Xue P; State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, 102206, China.
  • Zhou H; State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, 102206, China.
  • Sha D; Wuxi Center for Disease Control and Prevention, Jiangsu, 214023, China.
  • Wang D; State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, 102206, China.
  • Gao H; State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, 102206, China.
  • Li J; State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, 102206, China.
  • Diao B; State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, 102206, China.
  • Zhao H; State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, 102206, China.
  • Kan B; State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, 102206, China; Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Hangz
  • Zhang J; State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, 102206, China. Electronic address: zhangjingyun@icdc.cn.
Mol Cell Probes ; 55: 101689, 2021 02.
Article in En | MEDLINE | ID: mdl-33338586
ABSTRACT
A multiplex PCR (mPCR) assay was established to detect five pathogenic Vibrio species and Plesiomonas shigelloides. Twelve genes were included ompW, ctxA, rfbN, and wbfR from V. cholerae; tl, tdh, and trh from V. parahaemolyticus; toxR and vmhA from V. mimicus; toxR from V. fluvialis; vvhA from V. vulnificus; and the 23S rRNA gene from P. shigelloides. The specificity of the mPCR assay was 100% for the detection of 136 strains and the limits of detection (LoD) were 12.5-50 pg/reaction. The assay exhibited higher sensitivity than cultivation methods in the detection of APW cultures of 113 diarrhea samples. In the analysis of 369 suspected Vibrio populations from estuarine water samples, the specificity of the mPCR for V. cholerae and V. parahaemolyticus was 100% for both, while the sensitivities were 100% and 96.1%, respectively. The assay can be applied to screen enrichment cultures and suspected colonies from environmental and clinical samples.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Vibrio / Plesiomonas / Multiplex Polymerase Chain Reaction Type of study: Diagnostic_studies Limits: Humans Language: En Journal: Mol Cell Probes Journal subject: BIOLOGIA MOLECULAR / BIOTECNOLOGIA Year: 2021 Type: Article Affiliation country: China
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Vibrio / Plesiomonas / Multiplex Polymerase Chain Reaction Type of study: Diagnostic_studies Limits: Humans Language: En Journal: Mol Cell Probes Journal subject: BIOLOGIA MOLECULAR / BIOTECNOLOGIA Year: 2021 Type: Article Affiliation country: China