Biophysical phenotyping of mesenchymal stem cells along the osteogenic differentiation pathway.
Cell Biol Toxicol
; 37(6): 915-933, 2021 12.
Article
in En
| MEDLINE
| ID: mdl-33420657
ABSTRACT
Mesenchymal stem cells represent an important resource, for bone regenerative medicine and therapeutic applications. This review focuses on new advancements and biophysical tools which exploit different physical and chemical markers of mesenchymal stem cell populations, to finely characterize phenotype changes along their osteogenic differentiation process. Special attention is paid to recently developed label-free methods, which allow monitoring cell populations with minimal invasiveness. Among them, quantitative phase imaging, suitable for single-cell morphometric analysis, and nanoindentation, functional to cellular biomechanics investigation. Moreover, the pool of ion channels expressed in cells during differentiation is discussed, with particular interest for calcium homoeostasis.Altogether, a biophysical perspective of osteogenesis is proposed, offering a valuable tool for the assessment of the cell stage, but also suggesting potential physiological links between apparently independent phenomena.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Osteogenesis
/
Mesenchymal Stem Cells
Language:
En
Journal:
Cell Biol Toxicol
Journal subject:
TOXICOLOGIA
Year:
2021
Type:
Article
Affiliation country:
Italy