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Techniques for Characterizing Cytomegalovirus-Encoded miRNAs.
Diggins, Nicole L; Crawford, Lindsey B; Struthers, Hillary M; Hook, Lauren M; Landais, Igor; Skalsky, Rebecca L; Hancock, Meaghan H.
Affiliation
  • Diggins NL; Vaccine and Gene Therapy Institute, Oregon Health and Sciences University, Beaverton, OR, USA.
  • Crawford LB; Vaccine and Gene Therapy Institute, Oregon Health and Sciences University, Beaverton, OR, USA.
  • Struthers HM; Vaccine and Gene Therapy Institute, Oregon Health and Sciences University, Beaverton, OR, USA.
  • Hook LM; Vaccine and Gene Therapy Institute, Oregon Health and Sciences University, Beaverton, OR, USA.
  • Landais I; Vaccine and Gene Therapy Institute, Oregon Health and Sciences University, Beaverton, OR, USA.
  • Skalsky RL; Vaccine and Gene Therapy Institute, Oregon Health and Sciences University, Beaverton, OR, USA.
  • Hancock MH; Vaccine and Gene Therapy Institute, Oregon Health and Sciences University, Beaverton, OR, USA. hancocme@ohsu.edu.
Methods Mol Biol ; 2244: 301-342, 2021.
Article in En | MEDLINE | ID: mdl-33555594
ABSTRACT
microRNAs (miRNAs) are small noncoding RNAs that regulate gene expression at the posttranscriptional level by binding to sites within the 3' untranslated regions of messenger RNA (mRNA) transcripts. The discovery of this completely new mechanism of gene regulation necessitated the development of a variety of techniques to further characterize miRNAs, their expression, and function. In this chapter, we will discuss techniques currently used in the miRNA field to detect, express and inhibit miRNAs, as well as methods used to identify and validate their targets, specifically with respect to the miRNAs encoded by human cytomegalovirus.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Cytomegalovirus / MicroRNAs / Immunoprecipitation Limits: Humans Language: En Journal: Methods Mol Biol Journal subject: BIOLOGIA MOLECULAR Year: 2021 Type: Article Affiliation country: United States
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Cytomegalovirus / MicroRNAs / Immunoprecipitation Limits: Humans Language: En Journal: Methods Mol Biol Journal subject: BIOLOGIA MOLECULAR Year: 2021 Type: Article Affiliation country: United States