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High-definition imaging using line-illumination modulation microscopy.
Zhong, Qiuyuan; Li, Anan; Jin, Rui; Zhang, Dejie; Li, Xiangning; Jia, Xueyan; Ding, Zhangheng; Luo, Pan; Zhou, Can; Jiang, Chenyu; Feng, Zhao; Zhang, Zhihong; Gong, Hui; Yuan, Jing; Luo, Qingming.
Affiliation
  • Zhong Q; Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and Technology, Wuhan, China.
  • Li A; MoE Key Laboratory for Biomedical Photonics, School of Engineering Sciences, Huazhong University of Science and Technology, Wuhan, China.
  • Jin R; HUST-Suzhou Institute for Brainsmatics, Suzhou, China.
  • Zhang D; Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and Technology, Wuhan, China.
  • Li X; MoE Key Laboratory for Biomedical Photonics, School of Engineering Sciences, Huazhong University of Science and Technology, Wuhan, China.
  • Jia X; HUST-Suzhou Institute for Brainsmatics, Suzhou, China.
  • Ding Z; CAS Center for Excellence in Brain Science and Intelligence Technology, Chinese Academy of Science, Shanghai, China.
  • Luo P; Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and Technology, Wuhan, China.
  • Zhou C; MoE Key Laboratory for Biomedical Photonics, School of Engineering Sciences, Huazhong University of Science and Technology, Wuhan, China.
  • Jiang C; Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and Technology, Wuhan, China.
  • Feng Z; MoE Key Laboratory for Biomedical Photonics, School of Engineering Sciences, Huazhong University of Science and Technology, Wuhan, China.
  • Zhang Z; Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and Technology, Wuhan, China.
  • Gong H; MoE Key Laboratory for Biomedical Photonics, School of Engineering Sciences, Huazhong University of Science and Technology, Wuhan, China.
  • Yuan J; HUST-Suzhou Institute for Brainsmatics, Suzhou, China.
  • Luo Q; HUST-Suzhou Institute for Brainsmatics, Suzhou, China.
Nat Methods ; 18(3): 309-315, 2021 03.
Article in En | MEDLINE | ID: mdl-33649587
ABSTRACT
The microscopic visualization of large-scale three-dimensional (3D) samples by optical microscopy requires overcoming challenges in imaging quality and speed and in big data acquisition and management. We report a line-illumination modulation (LiMo) technique for imaging thick tissues with high throughput and low background. Combining LiMo with thin tissue sectioning, we further develop a high-definition fluorescent micro-optical sectioning tomography (HD-fMOST) method that features an average signal-to-noise ratio of 110, leading to substantial improvement in neuronal morphology reconstruction. We achieve a >30-fold lossless data compression at a voxel resolution of 0.32 × 0.32 × 1.00 µm3, enabling online data storage to a USB drive or in the cloud, and high-precision (95% accuracy) brain-wide 3D cell counting in real time. These results highlight the potential of HD-fMOST to facilitate large-scale acquisition and analysis of whole-brain high-resolution datasets.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Brain / Imaging, Three-Dimensional / Microscopy Language: En Journal: Nat Methods Journal subject: TECNICAS E PROCEDIMENTOS DE LABORATORIO Year: 2021 Type: Article Affiliation country: China
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Brain / Imaging, Three-Dimensional / Microscopy Language: En Journal: Nat Methods Journal subject: TECNICAS E PROCEDIMENTOS DE LABORATORIO Year: 2021 Type: Article Affiliation country: China