SP3 Protocol for Proteomic Plant Sample Preparation Prior LC-MS/MS.

Mikulásek, Kamil; Konecná, Hana; Potesil, David; Holánková, Renata; Havlis, Jan; Zdráhal, Zbynek

Mikulásek, Kamil; Konecná, Hana; Potesil, David; Holánková, Renata; Havlis, Jan; Zdráhal, Zbynek.

Affiliation

Mikulásek K; Mendel Centre for Plant Genomics and Proteomics, Central European Institute of Technology, Masaryk University, Brno, Czechia.
Konecná H; National Centre for Biomolecular Research, Faculty of Science, Masaryk University, Brno, Czechia.
Potesil D; Mendel Centre for Plant Genomics and Proteomics, Central European Institute of Technology, Masaryk University, Brno, Czechia.
Holánková R; National Centre for Biomolecular Research, Faculty of Science, Masaryk University, Brno, Czechia.
Havlis J; Mendel Centre for Plant Genomics and Proteomics, Central European Institute of Technology, Masaryk University, Brno, Czechia.
Zdráhal Z; Mendel Centre for Plant Genomics and Proteomics, Central European Institute of Technology, Masaryk University, Brno, Czechia.

Front Plant Sci ; 12: 635550, 2021.

Article in En | MEDLINE | ID: mdl-33777071

Key words

Arabidopsis thaliana; bottom-up; carboxylated magnetic beads; filter-aided sample preparation; mass spectrometry; protein cleanup; single-pot solid-phase-enhanced sample preparation; sodium dodecyl sulfate removal

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Full text: 1 Collection: 01-internacional Database: MEDLINE Language: En Journal: Front Plant Sci Year: 2021 Type: Article

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Full text: 1 Collection: 01-internacional Database: MEDLINE Language: En Journal: Front Plant Sci Year: 2021 Type: Article