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Lipid-Oriented Live-Cell Distinction of B and T Lymphocytes.
Kwon, Haw-Young; Kumar Das, Raj; Jung, Gun Tae; Lee, Hong-Guen; Lee, Sun Hyeok; Berry, Stuart N; Tan, Justin Kok Soon; Park, Solip; Yang, Jae-Seong; Park, Soohyun; Baek, Kangkyun; Park, Kyeng Min; Lee, Jae Won; Choi, Yun-Kyu; Kim, Ki Hean; Kim, Sangho; Kim, Kwang Pyo; Kang, Nam-Young; Kim, Kimoon; Chang, Young-Tae.
Affiliation
  • Kwon HY; Center for Self-Assembly and Complexity, Institute for Basic Science (IBS), Pohang 37673, Republic of Korea.
  • Kumar Das R; Department of Chemistry, National University of Singapore, 3 Science Drive 3, 117543, Singapore.
  • Jung GT; Department of Biomedical Science and Technology, Kyung Hee Medical Science Research Institute, Kyung Hee University, Seoul 02453, Republic of Korea.
  • Lee HG; Division of Advanced Materials Science, Pohang University of Science and Technology, Pohang 37673, Republic of Korea.
  • Lee SH; School of Interdisciplinary Bioscience and Bioengineering, Pohang University of Science and Technology (POSTECH), Pohang 37673, Republic of Korea.
  • Berry SN; Department of Chemistry, National University of Singapore, 3 Science Drive 3, 117543, Singapore.
  • Tan JKS; Department of Biomedical Engineering, National University of Singapore, 4 Engineering Drive 3, 117583, Singapore.
  • Park S; Computational Cancer Genomics Group, Structural Biology Programme, Spanish National Cancer Research Centre (CNIO), Madrid 28029, Spain.
  • Yang JS; Centre de Recerca en Agrigenòmica, Consortium CSIC-IRTA-UAB-UB, Cerdanyola del Vallès, 08193 Barcelona, Spain.
  • Park S; Department of Mechanical Engineering, Pohang University of Science and Technology, 77 Cheongam-Ro, Nam-gu, Pohang, Gyeongbuk 37673, Republic of Korea.
  • Baek K; Center for Self-Assembly and Complexity, Institute for Basic Science (IBS), Pohang 37673, Republic of Korea.
  • Park KM; Center for Self-Assembly and Complexity, Institute for Basic Science (IBS), Pohang 37673, Republic of Korea.
  • Lee JW; Department of Biochemistry, School of Medicine, Daegu Catholic University, 33, 17-gil, Duryugongwon-ro, Nam-gu, Daegu 42472, Republic of Korea.
  • Choi YK; Department of Applied Chemistry, Institute of Natural Science, Global Center for Pharmaceutical Ingredient Materials, Kyung Hee University, Yongin 446-701, South Korea.
  • Kim KH; Department of Chemistry, Pohang University of Science and Technology, Pohang 37673, Republic of Korea.
  • Kim S; Department of Mechanical Engineering, Pohang University of Science and Technology, 77 Cheongam-Ro, Nam-gu, Pohang, Gyeongbuk 37673, Republic of Korea.
  • Kim KP; Department of Biomedical Engineering, National University of Singapore, 4 Engineering Drive 3, 117583, Singapore.
  • Kang NY; Department of Biomedical Science and Technology, Kyung Hee Medical Science Research Institute, Kyung Hee University, Seoul 02453, Republic of Korea.
  • Kim K; Department of Applied Chemistry, Institute of Natural Science, Global Center for Pharmaceutical Ingredient Materials, Kyung Hee University, Yongin 446-701, South Korea.
  • Chang YT; Department of Convergence IT Engineering, Pohang University of Science and Technology, Pohang, Gyeongbuk 37673, Korea.
J Am Chem Soc ; 143(15): 5836-5844, 2021 04 21.
Article in En | MEDLINE | ID: mdl-33834782
The identification of each cell type is essential for understanding multicellular communities. Antibodies set as biomarkers have been the main toolbox for cell-type recognition, and chemical probes are emerging surrogates. Herein we report the first small-molecule probe, CDgB, to discriminate B lymphocytes from T lymphocytes, which was previously impossible without the help of antibodies. Through the study of the origin of cell specificity, we discovered an unexpected novel mechanism of membrane-oriented live-cell distinction. B cells maintain higher flexibility in their cell membrane than T cells and accumulate the lipid-like probe CDgB more preferably. Because B and T cells share common ancestors, we tracked the cell membrane changes of the progenitor cells and disclosed the dynamic reorganization of the membrane properties over the lymphocyte differentiation progress. This study casts an orthogonal strategy for the small-molecule cell identifier and enriches the toolbox for live-cell distinction from complex cell communities.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: B-Lymphocytes / T-Lymphocytes / Cell Membrane / Fluorescent Dyes Limits: Animals Language: En Journal: J Am Chem Soc Year: 2021 Type: Article
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: B-Lymphocytes / T-Lymphocytes / Cell Membrane / Fluorescent Dyes Limits: Animals Language: En Journal: J Am Chem Soc Year: 2021 Type: Article