Generation of soluble, disulfide-rich JEV NS1 protein recognizable by anti-NS1 antibodies through a simplified, in vitro refolding approach.

Chong, Hui Ying; Leow, Chiuan Yee; Leow, Chiuan Herng

Chong, Hui Ying; Leow, Chiuan Yee; Leow, Chiuan Herng.

Affiliation

Chong HY; Institute for Research in Molecular Medicine (INFORMM), Universiti Sains Malaysia, Glugor, Penang, Malaysia.
Leow CY; School of Pharmaceutical Science, Universiti Sains Malaysia, Glugor, Penang, Malaysia.
Leow CH; Institute for Research in Molecular Medicine (INFORMM), Universiti Sains Malaysia, Glugor, Penang, Malaysia. Electronic address: herng.leow@usm.my.

Int J Biol Macromol ; 185: 485-493, 2021 Aug 31.

Article in En | MEDLINE | ID: mdl-34174313

Subject(s)

Antibodies, Viral/metabolism; Encephalitis Virus, Japanese/isolation & purification; Escherichia coli/growth & development; Viral Nonstructural Proteins/genetics; Disulfides/chemistry; Encephalitis Virus, Japanese/immunology; Epitopes/immunology; Escherichia coli/classification; Escherichia coli/genetics; Inclusion Bodies, Viral/immunology; Inclusion Bodies, Viral/metabolism; Protein Engineering; Protein Refolding; Solubility; Transformation, Bacterial; Viral Nonstructural Proteins/chemistry; Viral Nonstructural Proteins/immunology; Viral Nonstructural Proteins/metabolism

Key words

E. coli expression system; Flavivirus NS1; Immunodiagnostic; Inclusion body; in vitro protein refolding

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Viral Nonstructural Proteins / Encephalitis Virus, Japanese / Escherichia coli / Antibodies, Viral Language: En Journal: Int J Biol Macromol Year: 2021 Type: Article Affiliation country: Malaysia

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