Image fusion of IR and optical microscopy for mapping of biomolecules in tissue.
Analyst
; 146(19): 5848-5854, 2021 Sep 27.
Article
in En
| MEDLINE
| ID: mdl-34498612
ABSTRACT
It is shown that a pixel-level image fusion technique can produce images that combine the spatial resolution of optical microscopy images of haematoxylin and eosin (H&E) stained tissue with the chemical information in Fourier transform infrared (FTIR) images. The fused images show minimal distortion and the higher spatial resolution of the H&E images overcomes the diffraction limit on the spatial resolution of the FTIR images. A consideration of the FTIR spectra of nucleic acids and collagen can explain the changes in contrast between non-cancerous oral epithelium and underlying stroma within fused images formed by combining an H&E stain of oral tissue with FTIR images of the tissue obtained at a number of wavenumbers.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Connective Tissue
/
Microscopy
Language:
En
Journal:
Analyst
Year:
2021
Type:
Article
Affiliation country:
United kingdom