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Evaluation of three different genomic regions for detection of bovine leukemia virus by real-time PCR.
Fonseca Júnior, Antônio Augusto; Ferreira, Luciana Rabello; Laguardia-Nascimento, Mateus; Rivetti Júnior, Anselmo Vasconcelos; Bueno, Bruna Lopes; Dos Reis, Jenner Karlisson Pimenta; Camargos, Marcelo Fernandes.
Affiliation
  • Fonseca Júnior AA; Laboratório Federal de Defesa Agropecuária de Minas Gerais, Avenida Rômulo Joviano, Centro, Pedro Leopoldo, Minas Gerais, 33600-000, Brazil. antonio.biomol@gmail.com.
  • Ferreira LR; Centro Universitário de Sete Lagoas, UNIFEMM, Sete Lagoas, Brazil. antonio.biomol@gmail.com.
  • Laguardia-Nascimento M; Laboratório Federal de Defesa Agropecuária de Minas Gerais, Avenida Rômulo Joviano, Centro, Pedro Leopoldo, Minas Gerais, 33600-000, Brazil.
  • Rivetti Júnior AV; Laboratório Federal de Defesa Agropecuária de Minas Gerais, Avenida Rômulo Joviano, Centro, Pedro Leopoldo, Minas Gerais, 33600-000, Brazil.
  • Bueno BL; Laboratório Federal de Defesa Agropecuária de Minas Gerais, Avenida Rômulo Joviano, Centro, Pedro Leopoldo, Minas Gerais, 33600-000, Brazil.
  • Dos Reis JKP; Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.
  • Camargos MF; Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.
Braz J Microbiol ; 52(4): 2483-2488, 2021 Dec.
Article in En | MEDLINE | ID: mdl-34595727
ABSTRACT
Bovine leukemia virus (BLV) is an oncogenic member of the genus Deltaretrovirus. BLV infects cattle worldwide and is responsible for significant economic losses. The objective of this study was to validate real-time quantitative PCR (qPCR) for the detection of BLV. After identification of the most efficient qPCR, the limits of detection, repeatability, and reproducibility were determined. The results indicate that qPCR can be easily reproduced between laboratories with high sensitivity. The test variation was low in samples from lesions suggestive of bovine leukosis or whole blood.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Enzootic Bovine Leukosis / Leukemia Virus, Bovine Type of study: Diagnostic_studies / Prognostic_studies Limits: Animals Language: En Journal: Braz J Microbiol Year: 2021 Type: Article Affiliation country: Brazil
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Enzootic Bovine Leukosis / Leukemia Virus, Bovine Type of study: Diagnostic_studies / Prognostic_studies Limits: Animals Language: En Journal: Braz J Microbiol Year: 2021 Type: Article Affiliation country: Brazil