Tissue clearing and 3D imaging in developmental biology.
Development
; 148(18)2021 09 15.
Article
in En
| MEDLINE
| ID: mdl-34596666
ABSTRACT
Tissue clearing increases the transparency of late developmental stages and enables deep imaging in fixed organisms. Successful implementation of these methodologies requires a good grasp of sample processing, imaging and the possibilities offered by image analysis. In this Primer, we highlight how tissue clearing can revolutionize the histological analysis of developmental processes and we advise on how to implement effective clearing protocols, imaging strategies and analysis methods for developmental biology.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Developmental Biology
/
Imaging, Three-Dimensional
Limits:
Animals
/
Humans
Language:
En
Journal:
Development
Journal subject:
BIOLOGIA
/
EMBRIOLOGIA
Year:
2021
Type:
Article
Affiliation country:
France