FITC characterization of a cathepsin B-responsive nanoprobe for report of differentiation of HL60 cells into macrophages.
J Pept Sci
; 28(3): e3371, 2022 Mar.
Article
in En
| MEDLINE
| ID: mdl-34608710
ABSTRACT
A cathepsin B (Cat B)-responsive optical nanoprobe is designed and prepared for report of HL60 differentiation into macrophage. A peptide sequence FRFK is linked to fluorescein (FITC) via the distant amino group of its lysine and N-terminated with acrylic acid (AA) to yield a molecular fluorescent probe AA-FRFK (FITC). The molecular probe is further embedded in poly(lactic-co-glycolic acid) (PLGA) to form a fluorescent nanoprobe AA-FRFK (FITC)@PLGA. The resultant optical nanoprobe is degradable by lysosomal Cat B, which is expressed in macrophages with a level of 5-10 times of that in HL60 cells. As a result, a significant decrease in fluorescence intensity is associated with the differentiation process of HL60 to macrophage and can be used as an indication of the differentiation process. The findings may pave a way toward the development of a universal in vitro labeling strategy of exogenous stem cells for report of in vivo cell differentiation by a dual-mode imaging modality involving optical imaging and magnetic resonance imaging.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Cathepsin B
/
Macrophages
Limits:
Humans
Language:
En
Journal:
J Pept Sci
Journal subject:
BIOQUIMICA
Year:
2022
Type:
Article
Affiliation country:
China