Photoswitchable Molecular Communication between Programmable DNA-Based Artificial Membraneless Organelles.
Angew Chem Int Ed Engl
; 61(14): e202117500, 2022 03 28.
Article
in En
| MEDLINE
| ID: mdl-35090078
ABSTRACT
Spatiotemporal organization of distinct biological processes in cytomimetic compartments is a crucial step towards engineering functional artificial cells. Mimicking controlled bi-directional molecular communication inside artificial cells remains a considerable challenge. Here we present photoswitchable molecular transport between programmable membraneless organelle-like DNA coacervates in a synthetic microcompartment. We use droplet microfluidics to fabricate membraneless non-fusing DNA coacervates by liquid-liquid phase separation in a water-in-oil droplet, and employ the interior DNA coacervates as artificial organelles to imitate intracellular communication via photo-regulated uni- and bi-directional transfer of biomolecules. Our results highlight a promising new route to assembly of multicompartment artificial cells with functional networks.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Organelles
/
Artificial Cells
/
Biomolecular Condensates
Language:
En
Journal:
Angew Chem Int Ed Engl
Year:
2022
Type:
Article
Affiliation country:
China