Oligonucleotide directed mutagenesis of cauliflower mosaic virus DNA using a repair-resistant nucleoside analogue: identification of an agnogene initiation codon.
Gene
; 41(2-3): 225-31, 1986.
Article
in En
| MEDLINE
| ID: mdl-3519365
ABSTRACT
Mutation of the initiation codon of the dispensible open reading frame, ORF VII, of cauliflower mosaic virus (CaMV) delayed the appearance of disease symptoms, but the mutants reverted with high frequency. This suggests a role of this start codon in viral expression. Oligonucleotide-directed mutagenesis, utilizing a novel, repair-resistant deoxyguanosine analogue, 2'-deoxy-7-deazainosine (dDI), highly improved the yield of mutants.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Oligodeoxyribonucleotides
/
DNA Repair
/
Genes, Viral
/
Mosaic Viruses
/
Mutation
Type of study:
Diagnostic_studies
Language:
En
Journal:
Gene
Year:
1986
Type:
Article