Sequencing SARS-CoV-2 genomes from saliva.
Virus Evol
; 8(1): veab098, 2022.
Article
in En
| MEDLINE
| ID: mdl-35542310
ABSTRACT
Genomic sequencing is crucial to understanding the epidemiology and evolution of Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Often, genomic studies rely on remnant diagnostic material, typically nasopharyngeal (NP) swabs, as input into whole-genome SARS-CoV-2 next-generation sequencing pipelines. Saliva has proven to be a safe and stable specimen for the detection of SARS-CoV-2 RNA via traditional diagnostic assays; however, saliva is not commonly used for SARS-CoV-2 sequencing. Using the ARTIC Network amplicon-generation approach with sequencing on the Oxford Nanopore MinION, we demonstrate that sequencing SARS-CoV-2 from saliva produces genomes comparable to those from NP swabs, and that RNA extraction is necessary to generate complete genomes from saliva. In this study, we show that saliva is a useful specimen type for genomic studies of SARS-CoV-2.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Language:
En
Journal:
Virus Evol
Year:
2022
Type:
Article
Affiliation country:
United States